• Component Description
• Eligible Sample
• Description of Laboratory Methodology
• Data Processing and Editing
• Laboratory Quality Assurance and Monitoring
• Analytic Notes
• References
• Codebook
  • SEQN - Respondent sequence number
  • WTSSPO2Y - SSPOLI_F 2 year weights
  • SSPV1 - Antibody against wild type polio virus 1
  • SSPV2 - Antibody against wild type polio virus 2
  • SSPV3 - Antibody against wild type polio virus 3

National Health and Nutrition Examination Survey

2009-2010 Data Documentation, Codebook, and Frequencies

Poliovirus Serotypes 1, 2, & 3 Antibodies - Serum (Surplus) (SSPOLI_F)

Data File: SSPOLI_F.xpt

First Published: August 2014

Last Revised: April 2022

Component Description

Antibodies to poliovirus serotypes 1, 2, and 3 assayed from stored serum samples from NHANES (2009-10)

Eligible Sample

All participants aged 6-49 years with available specimen and consent.

Description of Laboratory Methodology

Samples were tested in triplicate using a standard microneutralization assay for antibodies to poliovirus types 1, 2, and 3 according to established protocols at the Global Polio Specialized Laboratory, Centers for Disease Control and Prevention (CDC)[1,2]. Briefly, 80-100 CCID50 of each poliovirus serotype and two-fold serial dilutions of serum were combined and pre-incubated at 35°C for 3 hours before addition of HEp-2(C) cells. After incubation for 5 days at 35°C and 5% CO2, plates were stained with crystal violet and cell viability measured by optical density in a spectrophotometer. Each specimen was run in triplicate, with parallel specimens from one study subject tested in the same assay run, and the neutralization titers estimated by the Spearman-Kärber method [3] and reported as the reciprocal of the calculated 50% endpoint. A serum sample was considered positive if antibodies were present at ≥1:8 dilution. Specimens with antibody titers <1:8 were considered seronegative and specimens with titers >1:8 were considered seropositive.(1).

Data Processing and Editing

All data will be made publicly available.

Laboratory Quality Assurance and Monitoring

Each run contained multiple replicates of a reference antiserum pool starting at a 1:32 dilution to monitor performance variation.

Analytic Notes

Subsample Weights

Sample weights are required to analyze these data properly. Specific sample weights for this subsample are included in this data file and should be used when analyzing these data. When observations were removed from this data file, a new sample weight was created and  added to this data file (WTSSPO2Y). Please refer to the NHANES Analytic Guidelines and the on-line NHANES Tutorial for further details on the use of sample weights and other analytic issues.

References

• Karber G. Archiv fuer Experimentelle Pathologie und Pharmakologie. Archiv fuer Experimentelle Pathologie und Pharmakologie 1931;162:480-3.
  
• World health Organization. Expanded Program on Immunization, editor. Manual for the virological investigation of polio. WHO EPI/GEN/97.01.EPV1 1997.
• World health Organization. Report of a WHO informal consultation on polio neutralization antibody assays.
  

Codebook and Frequencies