National Health and Nutrition Examination Survey

1999-2004 Data Documentation, Codebook, and Frequencies

Autoantibodies - Immunofluorescence & Immunoprecipitation Analyses (Surplus) (SSANA_A)

Data File: SSANA_A.xpt

First Published: June 2011

Last Revised: July 2022

Note: The NHANES Biospecimen Program processes were reevaluated in 2021 and 2022 to monitor quality control after a procedural error was identified. This error did not pose any risk of participant disclosure. Addressing this error resulted in the removal of some records from various stored biospecimen data files between 1999 and 2018 that did not meet program standards. After a comprehensive review of all stored specimen datasets, this data file was modified to remove records (<5% of records) that were initially included in error. No data values were altered. The sample weights and overall estimates were compared between the original and revised files. The sample weights provided with the original data release are appropriate to use with this updated data file; therefore, no new survey weights were created. The overall estimates from these files were examined and are consistent with the previous file. However, not all possible analyses were performed.

See Revised Analytical Note for NHANES 1999-2004 Immunofluorescence and immunoprecipitation analyses of autoantibodies (Revised January 2012)

Component Description

IgG autoantibody testing of a subsample stored serum of specimens from NHANES (1999–2004) was conducted to estimate the prevalence and specificities of selected autoantibodies in the US population in 1999-2004.

Eligible Sample

One third subsample of participants aged 12 years and older from NHANES 1999-2004 in the Dioxin subsample who had stored sera (total of N = ~5,000 from NHANES 1999-2004).

Description of Laboratory Methodology

Immunofluorescence analysis
Testing of IgG autoantibodies to human cellular antigens was performed by the HEp-2 cell immunofluorescence assay using slides from INOVA Diagnostics, San Diego, CA (Cat # 508100) following the manufacturer’s instructions.

The cellular pattern of staining and its staining intensity from 0 to 4+ was described using a reference gallery per below. The determination of autoantibody titers from 1:80 to 1:1280 was performed by serial dilution on samples that showed a 3+ or greater nuclear and/or cytoplasmic immunofluorescence pattern.

Immunoprecipitation analysis
Testing of IgG autoantibodies to human cellular antigens was performed on samples that showed a 3+ or greater immunofluorescence staining using immunoprecipitation assays by a standard method (Reeves WH,et al., 2006)

Sera that showed known autoantibody specificities based on visible immunoprecipitated protein bands consistent with the combination of bands and their estimated size per the table below were recorded. Immunoprecipitated ribonucleic acid bands on stained gels were also recorded when they were needed to confirm the specificity. When necessary, the size and the pattern of migration were compared with reference sera (Reeves WH,et al., 2006).

Data Processing and Editing

Data Access: All data are publicly available.

Laboratory Quality Assurance and Monitoring

Immunofluorescence analysis
Serum samples were tested using established protocols outlined by manufacturer. A set of CDC autoimmune reference sera (12 different specificities) (Chan EKL et al., 2007) and 2 healthy control sera were coded and tested in a blinded fashion two times to verify consistent results throughout screening test: towards the beginning and when approximately half of the samples were tested.

Samples that showed discrepant results were repeated.

More than every 50th sample (2% of the total from NHANES 1999-2004) was automatically repeated to assess assay precision issues. Additional samples were repeated for the following reasons:
1) Titering of 3+/4+ positive samples (repeat, n=692)
2) Randomly selected samples for titering (and repeat, n=1035)
3) Other repeats are performed whenever there is any doubt for accuracy or technical issues. Samples repeated with 2 independent staining = 895; 3 independent staining = 194; 4 independent staining = 4.

Immunoprecipitation analysis
Serum samples were tested using the standard immunoprecipitation protocol (Reeves WH,et al., 2006). A set of CDC autoimmune reference sera (12 specificities) and 2 healthy controls were tested in a blinded fashion two times to verify consistent results throughout screening test: towards the beginning of the study and when approximately half of the samples were tested.

Samples that showed common bands were repeated in parallel side-by-side fashion for comparison with each other and with known controls as needed.

Assay reproducibility was initially assessed by random selection of the ANA 3+ or 4+ sera number of serum specimens tested and repeating the immunoprecipitation assay. The percentage of samples that were repeated was actually 15% (103/692) of the sera tested by IP.

Analytic Notes

The NHANES Survey Design:

The analysis of NHANES laboratory data should be conducted using the key survey design variables. The NHANES Demographic data files for each 2-year survey cycle contain core information on the survey and the survey design variables for strata and PSU (SDMVSTRA and SDMVPSU, respectively) which are recommended to be used in variance estimation. This laboratory data files may be linked to other NHANES data files using the unique survey participant identifier SEQN.

Weighting:

Special subsample sample weights WTANA6YR in the file are required to analyze the NHANES 1999-2004 ANA data. This is because the ANA data were analyzed using stored serum samples obtained from survey participants who had been selected from NHANES 1999-2004 Dioxin subsamples. The NHANES 1999-2004 ANA sample and the subsample weights were created for 6 years analysis and should be used to analyze all six years together.

Immunofluorescence analysis

A standard gallery of images showing the intensity of staining and the cellular patterns of reactivity is shown below and the following patterns were reported:

Nuclear patterns
1) homogeneous 2) speckles 3) centromere
4) nuclear dots 5) nuclear envelope 6) nucleolar

Cytoplasmic patterns
1) homogeneous 2) speckles 3) mitochondria-like
4) Golgi complex 5) centriole 6) GW Body
7) cytofilament 8) rods/rings 9) lysosomes

Mitotic cell patterns
1) homogeneous 2) speckles 3) spindle apparatus
4) mid-body 5) chromosomes

ANA staining intensity reference gallery Staining Signal Guidelines for Multiple Staining Patterns

**Nuclear Patterns**
Nuclear staining	Images	Description of staining patterns
Homogeneous		Uniform diffuse staining of the nucleus in interphase cells
Speckles		Speckles in the nucleus of interphase cells
Centromere		Discrete speckles (40-80/cell) in interphase cells and on metaphase plates
Nuclear dots		Countable discrete speckles PML bodies, Cajal bodies (coiled bodies)
Nuclear envelope		Homogeneous nuclear staining with greater intensity at its outer rim
Nucleolar		Homogeneous or speckled staining of the nucleolus

**Cytoplasmic Patterns**
Cytoplasmic staining	Images	Description of staining patterns
Homogeneous		Uniform diffuse staining of the cytoplasm in interphase cells
Speckles		Speckled staining of the cytoplasm
Mitochondria-like		Coarse granular filamentous staining
Golgi complex		Discontinuous speckled or granular perinuclear staining
Centriole		Distinct dots (2-4/cell) in cytoplasm and at the poles of mitotic spindle
GW body		Discrete dots in cytoplasm of interphase cells
Cytofilament		Cytoskeleton staining with variable intensity interphase cells
Rods/Rings		Distinct rod and ring structures throughout the cytoplasm
Lysosomes		Distinct and bright speckled staining irregularly distributed in the cytoplasm

**Mitotic cell patterns**
Mitotic cell staining	Images	Description of staining patterns
Homogeneous		Uniform diffuse staining of the mitotic cytoplasm
Speckles		Numerous speckles in cytoplasm of mitotic cells
Spindle Apparatus		The spindle fibers between the poles are stained in mitotic cells, associated with cone-shaped decoration of the mitotic poles
Mid-body		Staining of the intercellular bridge that connects daughter cells by the end of cell division, but before cell separation
Chromosomes		Diffuse staining of condensed chromatin in mitotic cells

Immunoprecipitation analysis
A standard set of images defining the immunoprecipitation patterns of reactivity is shown below and the specific reactivities that are reported are shown in the table below. Those samples that did not show any of these reactivities were reported as negative.

Analysis of protein components of autoantigens (12.5% SDS-PAGE). 35S-methionine labeled K562 cell extract was immunoprecipitated by prototype human autoimmune sera or normal human serum (NHS). Components of each autoantigen are indicated by red arrowheads.

Analysis of protein components of autoantigens (12.5% SDS-PAGE). ³⁵S-methionine labeled K562 cell extract was immunoprecipitated by prototype human autoimmune sera or normal human serum (NHS). Components of each autoantigen are indicated by red arrowheads.

Analysis of protein components of autoantigens (8% SDS-PAGE). 35S-methionine labeled K562 cell extract was immunoprecipitated by prototype human autoimmune sera or normal human serum (NHS). Components of each autoantigen are indicated by red arrowheads.

Analysis of protein components of autoantigens (8% SDS-PAGE). ³⁵S-methionine labeled K562 cell extract was immunoprecipitated by prototype human autoimmune sera or normal human serum (NHS). Components of each autoantigen are indicated by red arrowheads.

Analysis of RNA components of autoantigens. K562 cell extract from 107 cells was immunoprecipitated by prototype sera for anti-U3RNP, Jo-1, and normal human serum (NHS). RNA component was extracted and analyzed by urea-PAGE and silver staining. RNA components of each autoantigen are shown with arrowheads. Total RNA and the pattern by anti-Sm serum are shown as references.

Analysis of RNA components of autoantigens. K562 cell extract from 10⁷ cells was immunoprecipitated by prototype sera for anti-U3RNP, Jo-1, and normal human serum (NHS). RNA component was extracted and analyzed by urea-PAGE and silver staining. RNA components of each autoantigen are shown with arrowheads. Total RNA and the pattern by anti-Sm serum are shown as references.

**Identification of autoantibodies by protein and RNA immunoprecipitation**
Autoantibody Specificity	35S-methionine IP protein analysis	IP-RNA analysis silver staining	Definition
U1RNP	Y	ND	A characteristic set of UsnRNPs proteins, U1-70k, A (33kD), B’/B (29/28kD), C (22kD), D1/D2/D3 (16kD). E,F, and G without U5-200kD doublet
Sm	Y	ND	A characteristic set of UsnRNPs proteins as above with U5-200kD doublet
Ribosomal P	Y	ND	A characteristic set of proteins P0 (38kD), P1 (19kD), and P2 (17kD)
Ro60 (SS-A)	Y	ND	60kD protein
La (SS-B)	Y	ND	48kD protein
Su (ago2)	Y	ND	A set of 100/102kD doublet and 200kD proteins
Replication protein A	Y	ND	A set of 70kD, 32kD, and 14kD proteins
Jo-1 (histidyl-tRNA synthetase)	(Y)	Y	50kD protein and tRNA by RNA analysis
PL-7 (threonyl-tRNA synthetase)	Y	ND	80kD protein
PL-12 (alanyl-tRNA synthetase)	Y	ND	110kD protein
EJ (glycyl-tRNA synthetase)	Y	ND	75kD protein
OJ (isoleucyl-tRNA synthetase)	Y	ND	130kD, 150kD, 170kD proteins
SRP (signal recognition particle)	(Y)	Y	72/68kD, 54kD, 19kD, 14kD, 9kD proteins and 7SL RNA by RNA analysis
Ku	Y	ND	70kD and 86kD proteins
PM-Scl	Y	ND	110kD, 75kD, and a set of other proteins
Mi-2	Y	ND	240kD, 150kD proteins and several other proteins
Topoisomerase I (Scl-70)	Y	ND	110kD protein with a smear toward higher molecular weight due to phosphorylation
RNA polymerase I/III	Y	ND	A characteristic set of proteins. Two largest subunits of each (RNAP I: 190, 120kD, RNAP III (155, 135kD)
U3RNP (Fibrillarin)	(Y)	Y	34kD protein, nucleolar staining, and U3RNA by RNA analysis
NOR-90	Y	ND	93/95kD thick protein doublet

IP: immunoprecipitation; Y: Identity can be confirmed; (Y) identity suggested but requires RNA-IP to confirm; ND: not done

References

Chan EKL, Fritzler MJ, Wiik A, Andrade LE, Reeves WH, Tincani A, Meroni PL. AutoAbSC.Org - Autoantibody Standardization Committee in 2006. Autoimmun.Rev. 2007;6(8):577-80.
Reeves WH, Satoh M, Lyons R, Nichols C, Narain S: Detection of autoantibodies against proteins and ribonucleoproteins by double immunodiffusion, immunoprecipitation, and western blotting. In: Manual of Molecular and Clinical Laboratory Immunology, 7th edition. Edited by Rose NR, Hamilton RG, Detrick B, Reeves WH. Washington, D.C.: American Society of Microbiology Press; 2006: 1007-1018.

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:: SEQN
SAS Label:: Respondent sequence number
English Text:: Respondent sequence number.
Target:: Both males and females 12 YEARS - 150 YEARS

WTANA6YR - Six-year SSANA subsample weights

Variable Name:: WTANA6YR
SAS Label:: Six-year SSANA subsample weights
English Text:: Six-year SSANA subsample weights
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative
1715.213371 to 262789.6562	Range of Values	4532	4532
0	No Lab Result	0	4532
.	Missing	0	4532

SSCYT - total cytoplasm intensity

Variable Name:: SSCYT
SAS Label:: total cytoplasm intensity
English Text:: total cytoplasm intensity
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSNUC - total nucleus intensity

Variable Name:: SSNUC
SAS Label:: total nucleus intensity
English Text:: total nucleus intensity
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSTOT - total ANA intensity

Variable Name:: SSTOT
SAS Label:: total ANA intensity
English Text:: total ANA intensity
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SS80 - 1:80 dilution

Variable Name:: SS80
SAS Label:: 1:80 dilution
English Text:: 1:80 dilution
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	628	628
.	Missing	3904	4532

SS160 - 1:160 dilution

Variable Name:: SS160
SAS Label:: 1:160 dilution
English Text:: 1:160 dilution
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	628	628
.	Missing	3904	4532

SS320 - 1:320 dilution

Variable Name:: SS320
SAS Label:: 1:320 dilution
English Text:: 1:320 dilution
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	628	628
.	Missing	3904	4532

SS640 - 1:640 dilution

Variable Name:: SS640
SAS Label:: 1:640 dilution
English Text:: 1:640 dilution
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	628	628
.	Missing	3904	4532

SS1280 - 1:1280 dilution or higher

Variable Name:: SS1280
SAS Label:: 1:1280 dilution or higher
English Text:: 1:1280 dilution or higher
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	628	628
.	Missing	3904	4532

SSNUCPOS - nuclear nucleolar positive

Variable Name:: SSNUCPOS
SAS Label:: nuclear nucleolar positive
English Text:: nuclear nucleolar positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSNUCSIG - nuclear nucleolar signal

Variable Name:: SSNUCSIG
SAS Label:: nuclear nucleolar signal
English Text:: nuclear nucleolar signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSNUHOMP - nuclear homogenous positive

Variable Name:: SSNUHOMP
SAS Label:: nuclear homogenous positive
English Text:: nuclear homogenous positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSNUHOMS - nuclear homogenous signal

Variable Name:: SSNUHOMS
SAS Label:: nuclear homogenous signal
English Text:: nuclear homogenous signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSNUSPP - nuclear speckles positive

Variable Name:: SSNUSPP
SAS Label:: nuclear speckles positive
English Text:: nuclear speckles positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSNUSPS - nuclear speckles signal

Variable Name:: SSNUSPS
SAS Label:: nuclear speckles signal
English Text:: nuclear speckles signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSNUCENP - nuclear centromere positive

Variable Name:: SSNUCENP
SAS Label:: nuclear centromere positive
English Text:: nuclear centromere positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSNUCENS - nuclear centromere signal

Variable Name:: SSNUCENS
SAS Label:: nuclear centromere signal
English Text:: nuclear centromere signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSNUDOTP - nuclear dots positive

Variable Name:: SSNUDOTP
SAS Label:: nuclear dots positive
English Text:: nuclear dots positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSNUDOTS - nuclear dots signal

Variable Name:: SSNUDOTS
SAS Label:: nuclear dots signal
English Text:: nuclear dots signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSNUENVP - nuclear envelope positive

Variable Name:: SSNUENVP
SAS Label:: nuclear envelope positive
English Text:: nuclear envelope positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSNUENVS - nuclear envelope signal

Variable Name:: SSNUENVS
SAS Label:: nuclear envelope signal
English Text:: nuclear envelope signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSCYHOMP - cytoplasmic homogeneous positive

Variable Name:: SSCYHOMP
SAS Label:: cytoplasmic homogeneous positive
English Text:: cytoplasmic homogeneous positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSCYHOMS - cytoplasmic homogeneous signal

Variable Name:: SSCYHOMS
SAS Label:: cytoplasmic homogeneous signal
English Text:: cytoplasmic homogeneous signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 3	Range of Values	4532	4532
.	Missing	0	4532

SSCYSPP - cytoplasmic speckles positive

Variable Name:: SSCYSPP
SAS Label:: cytoplasmic speckles positive
English Text:: cytoplasmic speckles positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSCYSPS - cytoplasmic speckles signal

Variable Name:: SSCYSPS
SAS Label:: cytoplasmic speckles signal
English Text:: cytoplasmic speckles signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSCYMIP - cytoplasmic mitochondria-like positive

Variable Name:: SSCYMIP
SAS Label:: cytoplasmic mitochondria-like positive
English Text:: cytoplasmic mitochondria-like positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSCYMIS - cytoplasmic mitochondria-like signal

Variable Name:: SSCYMIS
SAS Label:: cytoplasmic mitochondria-like signal
English Text:: cytoplasmic mitochondria-like signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSCYGOP - cytoplasmic golgi complex positive

Variable Name:: SSCYGOP
SAS Label:: cytoplasmic golgi complex positive
English Text:: cytoplasmic golgi complex positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSCYGOS - cytoplasmic golgi complex signal

Variable Name:: SSCYGOS
SAS Label:: cytoplasmic golgi complex signal
English Text:: cytoplasmic golgi complex signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSCYCEP - cytoplasmic centriole positive

Variable Name:: SSCYCEP
SAS Label:: cytoplasmic centriole positive
English Text:: cytoplasmic centriole positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSCYCES - cytoplasmic centriole signal

Variable Name:: SSCYCES
SAS Label:: cytoplasmic centriole signal
English Text:: cytoplasmic centriole signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSCYGWP - cytoplasmic GW body positive

Variable Name:: SSCYGWP
SAS Label:: cytoplasmic GW body positive
English Text:: cytoplasmic GW body positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSCYGWS - cytoplasmic GW body signal

Variable Name:: SSCYGWS
SAS Label:: cytoplasmic GW body signal
English Text:: cytoplasmic GW body signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSCYCFP - cytoplasmic cytofilament positive

Variable Name:: SSCYCFP
SAS Label:: cytoplasmic cytofilament positive
English Text:: cytoplasmic cytofilament positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSCYCFS - cytoplasmic cytofilament signal

Variable Name:: SSCYCFS
SAS Label:: cytoplasmic cytofilament signal
English Text:: cytoplasmic cytofilament signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSCYRRP - cytoplasmic rods/rings positive

Variable Name:: SSCYRRP
SAS Label:: cytoplasmic rods/rings positive
English Text:: cytoplasmic rods/rings positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSCYRRS - cytoplasmic rods/rings signal

Variable Name:: SSCYRRS
SAS Label:: cytoplasmic rods/rings signal
English Text:: cytoplasmic rods/rings signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSCYLYP - cytoplasmic lysosomes positive

Variable Name:: SSCYLYP
SAS Label:: cytoplasmic lysosomes positive
English Text:: cytoplasmic lysosomes positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSCYLYS - cytoplasmic lysosomes signal

Variable Name:: SSCYLYS
SAS Label:: cytoplasmic lysosomes signal
English Text:: cytoplasmic lysosomes signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 3	Range of Values	4532	4532
.	Missing	0	4532

SSMIHOMP - mitotic homogeneous positive

Variable Name:: SSMIHOMP
SAS Label:: mitotic homogeneous positive
English Text:: mitotic homogeneous positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSMIHOMS - mitotic homogeneous signal

Variable Name:: SSMIHOMS
SAS Label:: mitotic homogeneous signal
English Text:: mitotic homogeneous signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSMISPP - mitotic speckles positive

Variable Name:: SSMISPP
SAS Label:: mitotic speckles positive
English Text:: mitotic speckles positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSMISPS - mitotic speckles signal

Variable Name:: SSMISPS
SAS Label:: mitotic speckles signal
English Text:: mitotic speckles signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSMISAP - mitotic spindle apparatus positive

Variable Name:: SSMISAP
SAS Label:: mitotic spindle apparatus positive
English Text:: mitotic spindle apparatus positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSMISAS - mitotic spindle apparatus signal

Variable Name:: SSMISAS
SAS Label:: mitotic spindle apparatus signal
English Text:: mitotic spindle apparatus signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSMIMBP - mitotic mid-body positive

Variable Name:: SSMIMBP
SAS Label:: mitotic mid-body positive
English Text:: mitotic mid-body positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSMIMBS - mitotic mid-body signal

Variable Name:: SSMIMBS
SAS Label:: mitotic mid-body signal
English Text:: mitotic mid-body signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSMICHP - mitotic chromosomes positive

Variable Name:: SSMICHP
SAS Label:: mitotic chromosomes positive
English Text:: mitotic chromosomes positive
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	4532	4532
.	Missing	0	4532

SSMICHS - mitotic chromosomes signal

Variable Name:: SSMICHS
SAS Label:: mitotic chromosomes signal
English Text:: mitotic chromosomes signal
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 4	Range of Values	4532	4532
.	Missing	0	4532

SSU1RNP - U1 small nuclear ribonucleoprotein

Variable Name:: SSU1RNP
SAS Label:: U1 small nuclear ribonucleoprotein
English Text:: U1 small nuclear ribonucleoprotein
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	628	628
.	Missing	3904	4532

SSSM - Sm antigen

Variable Name:: SSSM
SAS Label:: Sm antigen
English Text:: Sm antigen
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	628	628
.	Missing	3904	4532

SSRIBOP - ribosomal P protein

Variable Name:: SSRIBOP
SAS Label:: ribosomal P protein
English Text:: ribosomal P protein
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	628	628
.	Missing	3904	4532

SSROSSA - Ro/SS-A 60kDa antigen

Variable Name:: SSROSSA
SAS Label:: Ro/SS-A 60kDa antigen
English Text:: Ro/SS-A 60kDa antigen
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	628	628
.	Missing	3904	4532

SSLASSB - La/SS-B antigen

Variable Name:: SSLASSB
SAS Label:: La/SS-B antigen
English Text:: La/SS-B antigen
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	628	628
.	Missing	3904	4532

SSSUAR2 - Su antigen/Argonaute2 (Ago2) protein

Variable Name:: SSSUAR2
SAS Label:: Su antigen/Argonaute2 (Ago2) protein
English Text:: Su antigen/Argonaute2 (Ago2) protein
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	628	628
.	Missing	3904	4532

SSRPA - replication protein A

Variable Name:: SSRPA
SAS Label:: replication protein A
English Text:: replication protein A
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	628	628
.	Missing	3904	4532

SSJO_1 - Jo-1 antigen

Variable Name:: SSJO_1
SAS Label:: Jo-1 antigen
English Text:: Jo-1 antigen
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0	0	628	628
.	Missing	3904	4532

SSPL_7 - PL-7 antigen

Variable Name:: SSPL_7
SAS Label:: PL-7 antigen
English Text:: PL-7 antigen
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0	0	628	628
.	Missing	3904	4532

SSPL_12 - PL-12 antigen

Variable Name:: SSPL_12
SAS Label:: PL-12 antigen
English Text:: PL-12 antigen
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0	0	628	628
.	Missing	3904	4532

SSEJ - EJ antigen

Variable Name:: SSEJ
SAS Label:: EJ antigen
English Text:: EJ antigen
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0	0	628	628
.	Missing	3904	4532

SSOJ - OJ antigen

Variable Name:: SSOJ
SAS Label:: OJ antigen
English Text:: OJ antigen
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	628	628
.	Missing	3904	4532

SSSRP - signal recognition particle

Variable Name:: SSSRP
SAS Label:: signal recognition particle
English Text:: signal recognition particle
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0	0	628	628
.	Missing	3904	4532

SSKU - Ku antigen

Variable Name:: SSKU
SAS Label:: Ku antigen
English Text:: Ku antigen
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0	0	628	628
.	Missing	3904	4532

SSPM_SCL - PM-Scl antigen

Variable Name:: SSPM_SCL
SAS Label:: PM-Scl antigen
English Text:: PM-Scl antigen
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0	0	628	628
.	Missing	3904	4532

SSMI_2 - Mi-2 antigen

Variable Name:: SSMI_2
SAS Label:: Mi-2 antigen
English Text:: Mi-2 antigen
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0	0	628	628
.	Missing	3904	4532

SSTOPOI - Topoisomerase I

Variable Name:: SSTOPOI
SAS Label:: Topoisomerase I
English Text:: Topoisomerase I
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	628	628
.	Missing	3904	4532

SSRNAPOL - RNA polymerase I and III

Variable Name:: SSRNAPOL
SAS Label:: RNA polymerase I and III
English Text:: RNA polymerase I and III
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0	0	628	628
.	Missing	3904	4532

SSU3RNP - U3 small nuclear ribonucleoprotein

Variable Name:: SSU3RNP
SAS Label:: U3 small nuclear ribonucleoprotein
English Text:: U3 small nuclear ribonucleoprotein
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0	0	628	628
.	Missing	3904	4532

SSNOR_90 - nucleolar organizer region antigen 90kDa

Variable Name:: SSNOR_90
SAS Label:: nucleolar organizer region antigen 90kDa
English Text:: nucleolar organizer region antigen 90kDa
Target:: Both males and females 12 YEARS - 150 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0 to 1	Range of Values	628	628
.	Missing	3904	4532

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