• Component Description
• Eligible Sample
• Description of Laboratory Methodology
• Data Processing and Editing
• Laboratory Quality Assurance and Monitoring
• Analytic Notes
• Codebook
  • SEQN - Respondent sequence number
  • ORXGH - HPV High Globulin Band result
  • ORXGL - HPV Low Globulin Band result
  • ORXH06 - HPV Type 6
  • ORXH11 - HPV Type 11
  • ORXH16 - HPV Type 16
  • ORXH18 - HPV Type 18
  • ORXH26 - HPV Type 26
  • ORXH31 - HPV Type 31
  • ORXH33 - HPV Type 33
  • ORXH35 - HPV Type 35
  • ORXH39 - HPV Type 39
  • ORXH40 - HPV Type 40
  • ORXH42 - HPV Type 42
  • ORXH45 - HPV Type 45
  • ORXH51 - HPV Type 51
  • ORXH52 - HPV Type 52
  • ORXH53 - HPV Type 53
  • ORXH54 - HPV Type 54
  • ORXH55 - HPV Type 55
  • ORXH56 - HPV Type 56
  • ORXH58 - HPV Type 58
  • ORXH59 - HPV Type 59
  • ORXH61 - HPV Type 61
  • ORXH62 - HPV Type 62
  • ORXH64 - HPV Type 64
  • ORXH66 - HPV Type 66
  • ORXH67 - HPV Type 67
  • ORXH68 - HPV Type 68
  • ORXH69 - HPV Type 69
  • ORXH70 - HPV Type 70
  • ORXH71 - HPV Type 71
  • ORXH72 - HPV Type 72
  • ORXH73 - HPV Type 73
  • ORXH81 - HPV Type 81
  • ORXH82 - HPV Type 82
  • ORXH83 - HPV Type 83
  • ORXH84 - HPV Type 84
  • ORXHPC - HPV CP 6108
  • ORXHPI - HPV Type IS39
  • ORXHPV - Oral HPV Result

National Health and Nutrition Examination Survey

2009-2010 Data Documentation, Codebook, and Frequencies

Human Papillomavirus (HPV) - Oral Rinse (ORHPV_F)

Data File: ORHPV_F.xpt

First Published: October 2011

Last Revised: NA

Component Description

Estimate the prevalence and determinants of oral HPV infection in the United States population. Oral HPV infection is newly appreciated as a strong risk factor for a distinct type of oropharyngeal squamous cell carcinoma that is rising in incidence in the United States. It has been estimated that oral HPV16 infection confers an approximate 15-fold increase in risk for oropharyngeal cancer. Despite these strong risks, little is known about the epidemiology of oral HPV infection. No population-based surveys of oral HPV infection, type distribution and determinants have been performed in any population worldwide.

Eligible Sample

Examined participants, aged 14-69 years, were tested. The public data file includes data for persons 18-69 years of age. Please see Analytic Notes about the release of data for adolescents 14-17 years of age.

Description of Laboratory Methodology

Oral rinse specimens were processed, stored and shipped to the Ohio State University Gillison Laboratory, Columbus, Ohio. Detailed specimen collection and processing instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM).

Samples will be centrifuged at 4,000 x g for 10 minutes at 4°C, the pellet will be resuspended in 1 ml of Puregene cell lysis solution and incubated at 37°C for 15 min followed by DNase-free RNase A (5µg/ml) digestion for 30 min at 37°C. Proteinase K (20 mg/ml) digestion will be performed overnight at 55°C followed by heat inactivation for 10 min at 95°C. Protein precipitation solution (340 µL) will be added and sample will be further purified according to the Puregene DNA purification kit protocol.

Purified DNA will be analyzed for 37 types of HPV by means of a multiplex polymerase-chain-reaction (PCR) assay targeted to the conserved L1 region of the viral genome, using PGYM09/11 primer pools and primers for ß-globin (33). PCR products will be denatured in 0.13 N NaOH and hybridized to an immobilized HPV probe array (Roche Diagnostics) using an extended line-blot assay for genotyping of 37 HPV types, including HPV-6, -11, -16, -18, -26, -31, -33, -35, -39, -40, -42, -45, -51, -52, -53, -54, -55, -56, -57, -58, -59, -61, -62, -64, -66, -67, -68, -69, -70, -71, -72, -73, -81, -82 (MM4 and IS39 subtypes), -83, -84 and -89, and ß-globin (Roche Molecular Systems, Inc., Alameda, CA). Positive controls, consisting of 10 and 100 HPV-16 (SiHa) or HPV-18 (C4–2)-positive cells diluted in a background of HPV-negative cells (K562), and a negative control (K562 cells), in addition to the manufacturer’s controls, are included in each experiment. Samples positive for ß-globin will be considered of sufficient quality for analysis. The HPV type will be reported for positive samples.

Data Processing and Editing

Read the General Documentation on Laboratory Data file for detailed data processing and editing protocols.

Laboratory Quality Assurance and Monitoring

The NHANES quality control and quality assurance protocols (QA/QC) meet the 1988 Clinical Laboratory Improvement Act mandates. Detailed QA/QC instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM). Read the General Documentation on Laboratory Data file for detailed QA/QC protocols.

Analytic Notes

The analysis of NHANES laboratory data must be conducted with the key survey design and basic demographic variables. The NHANES Household Questionnaire Data Files contain demographic data, health indicators, and other related information collected during household interviews. They also contain all survey design variables and sample weights for these age groups. The phlebotomy file includes auxiliary information, such as the conditions precluding venipuncture. The household questionnaire and phlebotomy files may be linked to the laboratory data file using the unique survey participant identifier SEQN.

Exam sample weights should be used for analyses. Please refer to the NHANES Analytic Guidelines and the on-line NHANES Tutorial  for further details on the use of sample weights and other analytic issues.

The public release data file includes Oral HPV data for participants aged 18–69. Data for youth aged 14–17 years are available through the NCHS Research Data Center (RDC).

Codebook and Frequencies