Table of Contents

Component Description

Antibodies to poliovirus serotypes 1, 2, and 3 assayed from stored serum samples from NHANES (2009-10)

Eligible Sample

All participants aged 6-49 years with available specimen and consent.

Description of Laboratory Methodology

Samples were tested in triplicate using a standard microneutralization assay for antibodies to poliovirus types 1, 2, and 3 according to established protocols at the Global Polio Specialized Laboratory, Centers for Disease Control and Prevention (CDC)[1,2]. Briefly, 80-100 CCID50 of each poliovirus serotype and two-fold serial dilutions of serum were combined and pre-incubated at 35°C for 3 hours before addition of HEp-2(C) cells. After incubation for 5 days at 35°C and 5% CO2, plates were stained with crystal violet and cell viability measured by optical density in a spectrophotometer. Each specimen was run in triplicate, with parallel specimens from one study subject tested in the same assay run, and the neutralization titers estimated by the Spearman-Kärber method [3] and reported as the reciprocal of the calculated 50% endpoint. A serum sample was considered positive if antibodies were present at ≥1:8 dilution. Specimens with antibody titers <1:8 were considered seronegative and specimens with titers >1:8 were considered seropositive.(1).

Data Processing and Editing

All data will be made publicly available.

Laboratory Quality Assurance and Monitoring

Each run contained multiple replicates of a reference antiserum pool starting at a 1:32 dilution to monitor performance variation.

Analytic Notes

Subsample Weights

Sample weights are required to analyze these data properly. Specific sample weights for this subsample are included in this data file and should be used when analyzing these data. When observations were removed from this data file, a new sample weight was created and  added to this data file (WTSSPO2Y). Please refer to the NHANES Analytic Guidelines and the on-line NHANES Tutorial for further details on the use of sample weights and other analytic issues.

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 6 YEARS - 49 YEARS

WTSSPO2Y - SSPOLI_F 2 year weights

Variable Name:
WTSSPO2Y
SAS Label:
SSPOLI_F 2 year weights
English Text:
Surplus specimen SSPOLI_F 2 year weights
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
8827.298071 to 182929.05598 Range of Values 4254 4254
0 No lab specimen 0 4254
. Missing 0 4254

SSPV1 - Antibody against wild type polio virus 1

Variable Name:
SSPV1
SAS Label:
Antibody against wild type polio virus 1
English Text:
Antibody against wild type polio virus 1
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
2.5 to 10.5 Range of Values 4254 4254
. Missing 0 4254

SSPV2 - Antibody against wild type polio virus 2

Variable Name:
SSPV2
SAS Label:
Antibody against wild type polio virus 2
English Text:
Antibody against wild type polio virus 2
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
2.5 to 10.5 Range of Values 4254 4254
. Missing 0 4254

SSPV3 - Antibody against wild type polio virus 3

Variable Name:
SSPV3
SAS Label:
Antibody against wild type polio virus 3
English Text:
Antibody against wild type polio virus 3
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
2.5 to 10.5 Range of Values 4254 4254
. Missing 0 4254