Table of Contents

Component Description

Human papillomavirus (HPV) infection is one of the most common sexually transmitted infections in the United States. Cervical infection with certain types of HPV is a major risk factor for cervical cancer in women. No national surveillance system exists to measure the full burden of HPV infection, and no reliable national population estimate of HPV exists. NHANES offers a unique opportunity to assess the prevalence of HPV infection in the general population.

Reducing the prevalence of HPV infection is a Developmental Healthy People 2010 objective: “Reducing the number of new HPV cases can help minimize the overall number of cases of high risk subtypes associated with cervical cancer in females ...” An HPV vaccine has been licensed, and knowledge of the national prevalence of HPV infection is critical for planning vaccination strategies and monitoring the impact of vaccination in the United States.

Eligible Sample

All participants aged 14 to 17 years.

Description of Laboratory Methodology

Multiplex Luminex Assay of Antibodies to Neutralizing Epitopes on HPV 6, 11, 16 and 18 L1-Virus-Like Particles (VLPs)

This method uses the Luminex platform to simultaneously assay antibodies to HPV 6, 11, 16 and 18. Type-specific antibodies of any Ig class are detected by displacement of fluorescently tagged neutralizing monoclonal antibody from VLP-coated microspheres.

Data Processing and Editing

Blood specimens were processed, stored and shipped to Atlanta, Ga. for analysis. Detailed specimen collection and processing instructions are discussed in the NHANES LPM. Read the LABDOC file for detailed data processing and editing protocols. The analytical methods are described in the Description of the Laboratory Methodology section. The Multiplex Luminex assay was conducted at Merck Research Laboratories using their protocol established for vaccine clinical trials.

Analytic Notes

The analysis of NHANES laboratory data must be conducted with the key survey design and basic demographic variables. The Demographic file contains: Status Variables providing core information on the survey participant including examination status, Recoded Demographic Variables including age, gender, race etc., and Interview and Examination Sample Weight Variables and Survey Design Variables. The recommended procedure for variance estimation requires use of stratum and PSU variables (SDMVSTRA and SDMVPSU, respectively), which are included in the demographic data file for each data release. The Questionnaire Data Files contain socio-economic data, health indicators, and other related information collected during household interviews. The Phlebotomy Examination file includes auxiliary information on duration of fasting, the time of day of the venipuncture, and the conditions precluding venipuncture. The Demographic, Questionnaire and Phlebotomy Examination files may be linked to the laboratory data file using the unique survey participant identifier SEQN.

Serum

Competitive Luminex Assay for HPV 6, 11, 16, 18: Merck established the serostatus cut-off values for a positive result for each HPV type in the assay as listed below. A final titer below this value is negative and a value equal or above is considered positive.

Sero-Status Cutoff(mMU/mL): HPV6=20, HPV11=16, HPV16=20, HPV18=24.

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 14 YEARS - 17 YEARS

LBXS06MK - HPV 06 (Merck competitive Luminex assay)

Variable Name:
LBXS06MK
SAS Label:
HPV 06 (Merck competitive Luminex assay)
English Text:
HPV 06 (Merck competitive Luminex assay)
Target:
Both males and females 14 YEARS - 17 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 to 2 Range of Values 943 943
. Missing 154 1097

LBXS11MK - HPV 11 (Merck competitive Luminex assay)

Variable Name:
LBXS11MK
SAS Label:
HPV 11 (Merck competitive Luminex assay)
English Text:
HPV 11(Merck competitive Luminex assay)
Target:
Both males and females 14 YEARS - 17 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 to 2 Range of Values 943 943
. Missing 154 1097

LBXS16MK - HPV 16 (Merck competitive Luminex assay)

Variable Name:
LBXS16MK
SAS Label:
HPV 16 (Merck competitive Luminex assay)
English Text:
HPV 16 (Merck competitive Luminex assay)
Target:
Both males and females 14 YEARS - 17 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 to 2 Range of Values 943 943
. Missing 154 1097

LBXS18MK - HPV 18 (Merck competitive Luminex assay)

Variable Name:
LBXS18MK
SAS Label:
HPV 18 (Merck competitive Luminex assay)
English Text:
HPV 18 (Merck competitive Luminex assay)
Target:
Both males and females 14 YEARS - 17 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 to 2 Range of Values 943 943
. Missing 154 1097