Physicochemical
RNA, Single Strand
|
|
1
|
No
|
38-45(S)
|
protein 60-70%
|
lipid 20-25%
|
3-13%, RNA: 0.7-5.0%
|
5
|
M (MW 150-200 x 10\3/), G (64 x 10\3/), N (54 x 10\3/),
M (27 x 10\3/), NS (29-45 x 10\3/) (2,3)
|
0
|
|
1.18-1.20 in sucrose
|
625 (2)(S)
|
1.32 in CsCl
|
140(S)
|
|
| Stability of Infectivity (effects) |
Unstable at pH 3.0; stable in range pH 5-10
|
|
|
|
|
4.5 dex
|
>10.5 dex (1)
|
1:200
|
<4.0 dex
|
>9.0 dex (1)
|
Inactivated by ultraviolet light and x-radiation
|
|
| Virion Morphology |
Bullet-shaped (3)
|
180 x 75 nm
|
nm
|
nm
|
Electron microscopy
|
Surf. proj. 6-10 nm; bilayer lipid membrane (2)
|
Extended 3.5 nm; helical 30-35 turns; 49nm outer,
|
|
| Morphogenesis |
Cytoplasm (2)
|
Buds from plasma membrane
|
Extracellular and in cytoplasmic vesicles
|
Not usually
|
|
|
| Hemagglutination |
No
|
SMB ext. by sucrose-acetone + prot.
|
Goose**
|
6.0-6.8
|
|
|
24-37dC
|
Sucrose and acetone preparations with varying concentrations
of protamine sulfate tried unsuccessfully (1)
** Human O erythrocytes also tried.
|
CF, NT
|
Sucrose and acetone preparations with varying concentrations
of protamine sulfate tried unsuccessfully (1)
** Human O erythrocytes also tried.
|
| |
