| | | Despite five serial blind passages (ic) in newborn mice and hamsters, Chilibre virus did not produce illness or death in these animals. Attempts to produce CPE and CF antigen with Chilibre in MA-111, BHK-21, L-cells, LLC-MK2, primary mouse embryo, and primary RMK cells were also unsuccessful, thus CF tests were not performed. | | Cross-neutralization tests (plaque method) using Chilibre virus and hyperimmune hamster sera (hamsters immunized with infected Vero cells; homologous titer = 1024) were done against each of the following viruses and specific antisera with negative results: Naples, Punta Toro, Chagres, Icoaraci, Candiru, Itaporanga, Karimabad, Sicilian, Gabek Forest, Anhanga, Urucuri, Salehabad, Buenaventura, Frijoles, Nique, Caimito, Cacao, Aguacate, Charleville, Rift Valley fever, Alenquer, Gordil, Saint-Floris, Tehran, Toscana, and Itaituba. | | Slight neutralizing activity against Chilibre virus was noted with Pacui, Bujaru and Arumowot antisera as follows: | | | | Virus |
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| Antiserum | Chilibre | Pacui | Bujaru | Arumowot |
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| | * Reciprocal of highest serum dilution giving >90% plaque reduction . | | Chilibre | 1024 * | <16 | <16 | <16 | | Pacui | 64 | 4096 | <16 | <16 | | Bujaru | 16 | <16 | <16 | <16 | | Arumowot | 64 | <16 | <16 | 2048 |
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