| The following information was extracted from Casals [9] : | | | Antigen of registered Virus | | Immune serum of registered virus* |
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Immune Sera* | HI | | | HI | |
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| Ht/Ho | Ind | Antigens | Ht/Ho | Ind |
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| * Hyperimmune mouse sera | Chikungunya | N.D./1280+ | | Chikungunya | 40/10,240 | 1/256 | Mayaro | 40/1280 | 1/32 | Mayaro | 20/10,240 | 1/512 | Semliki | 40/2560 | 1/64 | Semliki | 20/10,240 | 1/512 | Sindbis | 80/1280 | 1/16 | Sindbis | 20/10,240 | 1/512 | WEE | 80/10,240 | 1/128 | WEE | 160/10,240 | 1/64 | VEE | 160/640+ | 1/4+ | VEE | 80/10,240 | 1/128 |
| The above data demonstrate the antigenic relationship of EEE with representative members of the group, using hyperimmune sera. There is generally less crossing by CF: the NT is most specific. For antigenic relationship to other members of Group A, consult their respective catalogue cards and see References [9] , [26] - [29] . | EEE virus has been antigenically classified as a distinct virus type and is considered not to be closely related to any of the other alphaviruses [30] . | North American and South American antigenic varieties of EEE virus exist in nature; and they usually are distinguished by specialized serological techniques [21] , [22] . | |
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