| | | Complement fixation and plaque-reduction neutralization test results with Gray Lodge viral antigen and antibody to some rhabdoviruses: | | | | Antibody a | Complement fixation | Plaque-reduction neutralization |
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| | a Hyperimmune mouse sera or asicitc fluid | | b Reciprocal of highest antibody dilution giving 3+ to 4+ complement-fixation or 80% reduction of a viral challenge of 60-70 PFU | | c Obtained from Dr. R. Emmons, California State Dept. of Public Health | | d Obtained from Dr. R. Shope, YARU | | e Obtained from CDC, Fort Collins, Colorado | | f Reference grouping rhabdoviral ascitic fluids obtained from NIAID. | | Gray Lodge | 16 b | 160 b | | Hart Park (AR70) | < 8 | < 5 | | Hart Park (V4038) c | 16 | 5 | | Flanders (R11987) d | < 8 | < 5 | | Flanders (63V162) e | 16 | 5 | | Grouping Ascitic Fluids f | | | Ployvalent 7 | < 8 | < 5 | | Polyvalent Rabies | < 8 | < 5 | | Group VSV | < 8 | < 5 |
| | No inhibition by BFN 3187 hyperimmune mouse serum (HIMS) of hemagglutination with WEE, SLE, POW, MOD, RB, CE, LOK, MD, BUT TUR, and VEE antigens. No indirect fluorescent antibody (IFA) was observed between BFN 3187 infected Vero cells and 1:5 dilutions of polyvalent grouping mouse ascitic fluids for Group C, Group Guama, Group Simbu, Group VSV, Group Bunyamwera, Group Califor- nia, Group Tacaribe, Group Phlebotomus fever, Group A, Polyvalent Quaranfil, Polyvalent Anopheles A, Polyvalent Bwamba, Polyvalent Patois, Group Capim, Group B, Polyvalent 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, and Polyvalent Rabies. Plaque-reduction neutralization tests with BFN 3187 antigen against the above polyvalent grouping fluids were also negative (BFN 3187 homologous titer was 160). By CF test, BFN 3187 antigen did not react with polyvalent grouping fluids for Group VSV, Polyvalent Rabies, and Polyvalent 7, whereas the homologous reaction with HIMS was 16. BFN 3187 antigen reacted at low titers [16] with HIMS to 2 of 4 Hart Park/Flanders viral strains in CF tests where homologous titers to Hart Park/Flanders strains ranged from 250 to <1024. | | Since registration of Gray Lodge virus, it was tested by by CF against 36 other known rhabdoviruses, including members of the Hart Park serogroup, and it did not cross-react with any of the other rhabdoviruses [2] . Subsequently, antibody to Gray Lodge virus was tested against 50 known rhabdo- viruses without any evidence of cross-reaction [3] . On the basis of these studies, Gray Lodge virus was omitted from the Hart Park serogroup (ed.). | | |
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