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  Las Maloyas   LMV

Possible Arbovirus

No

2

Placed at this biosafety level based on close antigenic or genetic relationship to other viruses in a group of 3 or more viruses, all of which are classified at this level.
Other Information
 

Anopheles A
Section V - Antigenic Relationship and Lack of Relationship to Other Viruses
 
Sucrose-acetone antigen of Las Maloyas virus was tested by CF with the following immune mouse ascitic fluids with negative results [4] .
Polyvalent 1 (BAH, BER, EgAn 1398-61, MTR, MTY, TETE), Polyvalent 2, ( BLM, GAM, JUR, MARU 11079, MNT), Polyvalent 4 (GA, NYM, THO, UUK), Polyvalent 5 (HUG, LS, MAT, SAW, SOL), Polyvalent 6 (CHO, MCO, PAC, TIM), Polyvalent 7 (FLA, HP, KC, KLA, MEB), Polyvalent 8 (BLU, CGL, CTF, EHD, IbAr 22619, IRI), Polyvalent 10 (DGK, DHO, UPO, WAN), Polyvalent 12 (BIA, OLI, OKO, TAT, WIT), Bunyamwera group, Theiler's GD VII, Simbu group, broad group A, broad group B, broad group C, Guama group, Capim group, California group, Phlebotomus fever group, Patois group, Tacaribe group, Polyvalent Vesicular Stomatitis, mouse hepatitis, TB4-1054, Kwatta, Barur, Aruac, CayAr 404, Triniti, Pacora, and Navarro.
Titer of 64 was observed with Anopheles group immune fluid that contains antibody to ANA, LUK, TCM, CoAr 1071, CoAr 3624 [3] , ANB, BOR, TUR, UMB, AND MPO. Additional CF test showed that Las Maloyas was not related to the Anopheles B or Turlock group viruses represented in that immune fluid.
Cross-CF test and cross-serum dilution plaque-reduction neutralization tests show Las Maloyas virus to belong to the Anopheles A complex of the ANA serogroup [1] , [2] , but to be a distinct member.
Results of cross-box CF and serum dilution plaque-reduction neutralization tests with strain AG80-24 and four Anopheles A serogroup viruses.
 
CF Titer of Antibody to: PRNT Titer of Antibody to:
Virus or Antigen AG80-24 ColAn 57389 LUK ANA CoAr 3624 AG80-24 ColAn 57389 LUK ANA CoAr 3624


- = <8, CF; <20, PRNT
AG80-24 64 256 128 - 8 >1280 - - - -
ColAn 57389 64 128 64 - - - 1280 - - -
Lukuni 16 32 >1024 - - - - >2560 - -
Anopheles A 16 32 128 64 128 - - - 640 -
CoAr 3624 - - - 32 128 - - - - >1280
 
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