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  Arbia   ARBV

Probable Arbovirus

No

3

Isufficient experience with virus; i.e., experience factor from SALS surveys was less than 500 in laboratory facilities with low biocontainment.
Other Information
 

Phlebotomus Fever
Section V - Antigenic Relationship and Lack of Relationship to Other Viruses
 
ISS.Phl.18 antigen and MIAF did not react in CF test with antigens and MIAF prepared against Toscana , SFN, and SFS viruses.
At YARU [2] ; ISS.Phl.18 was screened by indirect fluorescent antibody test against the following MIAF: Phlebotomus fever group, Toscana , SFN, Arumowot, Gordil, Saint Floris, SFS, Rift Valley Fever , Karimabad, Salehabad, Gabek Forest, VSV group, Isfahan , Chandipura , and Jug Bogdanovac. Positive reactions were obtained with Toscana , SFN, Gordil, Saint Floris, Salehabad, Gabek Forest, and the Phlebotomus fever group MIAF. ISS.Phl.18 antigen and MIAF were then tested by CF against Salehabad, Gabek Forest, Gordil, Toscana , SFN, Saint Floris antigens, and MIAF. Positive reactions were obtained between ISS.Phl.18, Salehabad, and Gabek Forest.
 
ANTIGEN MIAF
ISS.Phl.18 Salehabad Gabek Forest


a Serum titer/antigen titer.
ISS.Phl.18 256/> 256 a 128/>64 <4/<4
Salehabad 64/>128 256/> 128 <4/<4
Gabek Forest 16/4 <4/<4 >256/> 128
Cross NT tests (plaque reduction method) between ISS.Phl.18 and Salehabad viruses and MIAF gave the following results:
 
VIRUS MIAF
ISS.Phl.18 Salehabad


b Dilution producing >90% plaque reduction.
ISS.Phl.18 160 b <10
Salehabad <10 320
 
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