| | | Complement-fixation test: | | Grouping immune sera or ascitic fluids (in dilutions beginning at 1:4) for the listed groups, reacted negatively against antigen Mono Lake at dilution 1:8: Groups A, B, C, Anopheles A, Bunyamwera, Bwamba, California, Capim, Guama, Nyando, Palyam, rhabdoviruses, Simbu, Tacaribe, Tete, and VSV. | | The following type specific sera diluted as above, reacted negatively with Mono Lake antigen: Aruac, Changuinola, Cotia, Kern Canyon, LCM, Lukuni, Nariva, Pacui, rabies, and Tembe. | | Immune serum for Mono Lake with a titer of 128, reacted negatively with the following antigens: Acara, Aruac, Hart Park, Jurona, Lebombo, Mapputta, Marco, Mirim, Mossuril, Navarro, Nyando, Pacui, Palyam, and Triniti. | | In cross complement-fixation tests with about 40 non-group B tick-borne viruses, Mono Lake has only cross-reacted with members of the Kemerovo group. An example of the crossing is shown: | | | | Complement-fixation Test |
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| | Serum |
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| Antigen | KEM | LIP | TRB | CNU | ML | HUA | WM |
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| | Serum titer/antigen titer; first dilution = 1:4. | | Kemerovo | 512/128 | 64/32 | 64/32 | 8/8 | 16/32 | 0 | 0 | | Lipovnik | 64/64 | 256/64 | 128/64 | 8/8 | 8/8 | 0 | Traces | | Tribec | 64/256 | 128/256 | 128/256 | 8/32 | 16/128 | 0 | Traces | | Chenuda | 16/16 | 32/32 | 32/32 | 256/128 | 64/64 | 16/8 | Traces | | Mono Lake | 16/16 | 8/16 | 32/16 | 32/16 | 256/64 | 64/32 | | Huacho | 8/8 | | | 16/16 | 128/32 | 256/32 | | Wad Medani | 0 | | 8/16 | | | | 128/64 |
| | | | Neutralization Test |
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| | Virus |
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| Serum | Mono Lake | Huacho |
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| | Virus titer in dex, in the presence of the serum; ic test in mice. | | Mono Lake | <1.5 | 3.6 | | Huacho | 2.5 | <1.5 | | Normal mouse | 5.1 | 5.2 |
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