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Virus Name:
Nodamura
Abbreviation:
NODV
Status
Probably not Arbovirus
Select Agent
No
SALS Level
3
SALS Basis
Isufficient experience with virus; i.e., experience factor from SALS surveys was less than 500 in laboratory facilities with low biocontainment.
Other Information
Antigenic Group
Ungrouped
Section IX - Experimental Arthropod Infection and Transmission
Arthropod species & virus source(a)
Method of Infection log10/ml (b)
Incubation period (c)
Transmision by bite (d)
Assay of arthropod, log10/ml (e)
Feeding
Injected
Days
°C
Host
Ratio
Whole
Organ
System
Dr. H. Hurlbut has passed Mag 115 virus serially by inoculation of mosquitoes (Culex tarsalis), ticks (Ornithodoros savigni), moth larvae (Plodia interpunctella) and beetle larvae (Attagenus piceus). Virus did not pass in bed bugs (Cimex lectularius) or cockroaches (Blattella germinica). Virus multiplies in bodies of Ae aegypti; no growth in heads. Virus transmitted to suckling mice by Ae aegypti 1-11 days after feeding on viremia mice.
NOD virus kills ad honey bees by 7 days at 30-35C; recover 33 nm virus particles. Kills wax moth larvae 7-14 days 30C (5). NOD virus mult., caused paralysis, death following intrathoracic inoc. into ad Ae albopictus and Toxorhynchites amboinensis mosquitoes. Mult. poorly or not at all in similarly injected Cx quinquefasciatus mosquitoes. Virus mult. in but did not kill Ae albopictus mosquitoes infected by feeding or immersion. Indirect IFA showed NOD antigen in brains. Ae pseudoscutellaris (AP61) cell cultures more sensitive than intracranial inoc. of nb mice for detection of NOD virus (10).
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