| | | Initially, CF tests carried out at YARU by GMW showed relationship with Turlock group. Cross tests indicated identity with Umbre virus, but subsequently with higher titered immune ascitic fluid at JCSMR, identity was not confirmed. Cross neutralization tests at JCSMR indicated that BH2193/p2 is readily distinguish- able from Umbre prototype IG 1424/R620/p5. | | Subsequently, plaque-purified Barmah Forest virus was shown to be related to alphaviruses by the HI test only. Barmah Forest virus did not react with any of the alphaviruses by CF or NT [1] , [2] . Interestingly, Umbre HA was inhibited in the HI test by antibody to Barmah Forest virus; and Umbre virus reacted ""weakly"" in the CF test with Barmah Forest virus. However, data obtained with regard to the mode of replication, morphology and morphogenesis, structural components of the virus and sequence homologies between the proteins of Barmah Forest virus and other alphaviruses have permitted the conclusion that Barmah Forest virus is an alphavirus with some apparently unusual features [1] . | | In a separate study, BF virus was tested against 18 additional or a total of 24 alphaviruses by HI, CF and NT. Cross-reactions were not detected by CF and NT. Low level (titers of 10-80) cross-reactions were observed in the HI test, and with one exception, only when BF antigen reacted with antibodies to several of the various alphaviruses. In one instance, SF antigen was inhibited by BF antibody to a titer of 10 [2] . | | | | Hemagglutination-Inhibition Tests with Barmah Forest Virus [1] |
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| | Hemagglutinin |
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| Immune Ascitic Fluid | BFV | SFV | SIN | RRV | BEB | UMB |
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| | a Reciprocal of dilution endpoint. | | b No reaction at 1:10 dilution of immune ascitic fluid. | | BFV | 640 a | - b | - | - | - | - | | Murweh | 320 | | | | | | Semliki Forest | 20 | 240 | | | | | Sindbis | 80 | 30 | 960 | | | - | | Whataroa | 30 | | | | | | Ross River | 10 | 15 | | 80 | | - | | Getah | - | | | | | | Bebaru | 20 | | | | 60 | - | | Umbre | 20 | - | - | - | - | 10240 | | Turlock | - | | | | | | Normal | - | - | - | - | - | - |
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