Physicochemical
RNA, Single Strand
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3
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(S)
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4 (20)
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Determined by polyacrylamide gel electrophoresis;designated VP-1, VP-2, VP-3, and VP-4,with MW of 17,500, 30,000,38,000, and 82,000 respectively. VP-2, VP-3 and VP-4 wereglycoproteins apparently associated with viral envelope (20).
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1.18 gm/ml (21) in sucrose
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350 S (21)(S)
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1.26 gm/ml (21)
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(S)
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| Stability of Infectivity (effects) |
Reasonably stable pH 7-9 at 4dC (21)
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50%
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<1.0 dex
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5.3 dex (3)
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1:1000
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<1.0 dex
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5.8 dex (3)
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1:1000 after treatment titer reduced 2.1 dex (4)
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| Virion Morphology |
Spherical
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98 nm (2); 50 nm (5)
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nm
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nm
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Electron microscopy
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| Morphogenesis |
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Found in cytoplasm in cell culture and mouse brain
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| Hemagglutination |
Yes
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SMB ext. by sucrose-acetone
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Goose
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5.8-6.6
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6.0, 6.2
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24 - 37dC
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24 or 37dC
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Titer with virus strain BFS 283 usually 160 to 640.
Some other strains produce poorer or no HA; enhanced by sonication.
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CF, NT, HI
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Titer with virus strain BFS 283 usually 160 to 640.
Some other strains produce poorer or no HA; enhanced by sonication.
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