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| | Chikungunya Antigen | Chikungunya Immune Serum |
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| | HI | CF | NT | HI | CF | NT |
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| | Ht/HO | Ratio | Ht/Ho | Ratio | ic | Ht/Ho | Ratio | Ht/Ho | Ratio | ic |
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| | * NT done with the Th-35 strain (Thailand); NT results given as LNI in dex. | | All immune sera were prepared in mice by repeated inoculations. | | ONN | 20/2560 | 1/128 | <8/128 | >1/16 | 2.5* | 160/320 | 1/2 | 64/256 | 1/4 | 4.4* | | Mayaro | 160/5120 | 1/32 | 8/32 | 1/4 | 2.2 | 80/2560 | 1/32 | 16/128 | 1/8 | 2.7 | | Semliki | 40/5120 | 1/128 | <4/256 | >1/64 | 1.4 | <10/2560 | >1/256 | 16/128 | 1/8 | 2.0* | | WEE | 12/3870 | 1/320 | <4/128 | >1/32 | 0.6 | 16/2080 | 1/130 | <4/128 | >1/32 | | EEE | 15/5490 | 1/360 | <4/512 | >1/128 | 0.4 | 7/2080 | 1/300 | <4/128 | >1/32 | | VEE | 14/4780 | 1/340 | <4/512 | >1/128 | 0.8 | 7/2080 | 1/300 | <4/128 | >1/32 | | Sindbis | 8/968 | 1/120 | <4/256 | >1/64 | 0 | 8/2080 | 1/300 | <4/128 | >1/32 | <1.8* | | AMM2021 | 20/10240 | 1/512 | 8/256 | 1/32 | | 49/2080 | 1/42 | <4/128 | >1/32 | <1.3* |
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| African strains appear to be antigenically more primitive than Asian strains [26] and can be clearly distinguished. |
| Plaque inhibition tests show (a) chikungunya and o'nyong-nyong can be distinguished only by using single-inoculation mouse antisera, not multiple- inoc. mouse, or rabbit, (b) African and Asian strains of chikungunya are not separable using mouse antisera only, (c) Semliki Forest antiserum inhibits chikungunya plaques, but not vice versa. |
| SIRACA has antigenically classified chikungunya virus and placed it in the Semliki Forest complex of serogroup A. In addition, ONN virus has been classified as a subtype of chikungunya virus [14] . For additional information on antigenic relationships, see References [17] , [39] - [42] . |
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