| | | Related to other Simbu Group viruses by CF (Filippich, personal communi- cation, QIMR). | | Douglas virus was placed within the Simbu complex of the Simbu serogroup primarily by CF testing [2] . Other members of this complex consisted of Simbu, Akabane, Yaba-7, Shamonda, Sabo, Sango, Peaton, Aino, Shuni, Kaikalur, Sathuperi, and Tinaroo viruses. | | Neutralization testing indicated that Douglas virus was most closely related to Sathuperi virus and distantly related to Shuni virus. Douglas virus was considered to be a subtype of Sathuperi virus [2] . | | Results of serum dilution plaque-reduction neutralization tests [2] : | | | | Titer of antibody to: |
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| Virus | Douglas | Sathuperi | Shuni |
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| | * 90% plaque-reduction titers expressed as the geometric mean of two or more determinations. | | Douglas | 1280 * | 920 | 10 | | Sathuperi | 1280 | 5120 | 20 | | Shuni | <10 | 120 | 11760 |
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