| | | Studies at the Virus Research Centre, Poona [1] : | | No relationship was detected by the CF test with immune sera to the following viruses: Alphaviruses; CHIK, SIN. Flaviviruses; JE, WN, DEN, types 1, 2, 3, and 4, KFD, MVE, NTA, and LGT. Bunyaviruses; UMB, BAT, and KSO. Orbiviruses; AHS, type 2, and PAL. Rhabdovirus; CHP. Herpesvirus; herpes simplex. Reovirus; reovirus type 3. Orthomyxoviridae; DHO. Unclassified: VKT, Ichampadi, ARK, and MIN. In preliminary CF tests, relationship was established between VRC 713423-2 and Sathuperi virus. | | The results of neutralization tests in infant mice with 713423-2, Sathuperi and Ingwavuma viruses and the respective homologous and heterologous immune sera were as follows: | | | | Virus |
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| Immune sera | 713423-2 | Sathuperi | Ingwavuma |
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| | Titre | LNI a | Titre | LNI | Titre | LNI |
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| | a LNI in dex. | | 713423-2 | <2.0 | > 4.5 | > 6.7 | +1.0 | 6.5 | 0.2 | | Sathuperi | 4.2 | 2.3 | 2.7 | > 5.0 | ND | | Normal mouse serum | 6.5 | | > 7.7 | | 6.7 | | BAPS control titrations | 6.0 | | > 7.6 | | 6.6 |
| | Studies at Yale University School of Medicine: | | Neutralization tests in Vero cells were conducted with 713423-2 virus and mouse immune ascitic fluid (MIAF) to the following viruses belonging to the Simbu group: AKA, BUT, ING, MAN, MER, ORO, SABO, SAN, SHA, SHU, SIM, THI, Utinga, and Yaba 7. 713423-2 was neutralized only by MIAF to Shuni virus. The relationship between 713423-2 and Shuni and Sathuperi viruses is shown by the following results of neutralization tests in Vero cells: | | | | | Virus |
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| | 713423-2 | Shuni | Sathuperi |
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| MIAF | Virus dose (dex) | Titre b | LNI c | Virus dose (dex) | Titre | LNI | Virus dose (dex) | Titre | LNI |
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| | b Serum dilution neutralization endpoints vs. 100 TCD50. | | c Constant serum-varying virus dilution neutralization indices in dex. | | 713423-2 | 1.8 | 320 | >4.35 | 1.5 | 240 | >4.0 | 2.3 | 60 | 2.5 | | Shuni | 1.5 | 210 | 3.0 | 2.0 | >1280 | >4.0 | 1.7 | 90 | 3.7 | | Sathuperi | 1.8 | 80 | 2.85 | 2.0 | 70 | 3.0 | 2.0 | >1280 | >4.3 |
| | 713423-2 is closely related to Shuni virus but the sixfold or higher difference in titres of Shuni MIAF when tested with Shuni and 713423-2 viruses, respectively, indicate that they are not identical. | | |
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