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Virus Name: Palestina

Abbreviation: PLSV

Status
Probable Arbovirus

Select Agent
No

SALS Level
3

SALS Basis
Isufficient experience with virus; i.e., experience factor from SALS surveys was less than 500 in laboratory facilities with low biocontainment.

Other Information

Antigenic Group
Minatitlan

Section V - Antigenic Relationship and Lack of Relationship to Other Viruses

Antigen and antibody for strain 76V-1565 were tested by complement-fixation with individual and grouping immune mouse ascitic fluids (MIAF) or antigens for more than 250 recognized arboviruses. Antigen of 76V-1565 reacted at 1:32 with NIH Polyvalent no. 2 MIAF. This MIAF contained antibody to Minatitlan, Jurona, Alajuela, Gamboa and Belem viruses. Subsequent CF tests with 76V-1565 antigen gave the following results:

	CF titer with:
Antibody to:	PLS Antigen	Homologous


Minatitlan	64	128
Mirim	16	128
Patois	16	64
Capim	8	128
Guama	8	512
Belem	<8	32
Jurona	<8	64
Gamboa	<8	64
Alajuela	<8	32

Cross-reactivity of strain 76V-1565 and Minatitlan and Mirim viruses by CF and serum dilution plaque-reduction neutralization tests.

MIAF
	CF			NT
Antigen or Virus	76V-1565	MNT	MIR	76V-1565	MNT	MIR


^b Reciprocal of highest dilution producing >90% plaque-reduction.
76V-1565	64	128	8	160 ^b	<20	<20
Minatitlan	16	256	8	40	2560	<20
Mirim	8	8	256	<20	<20	320

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