| | | Serological relationships between Prospect Hill virus, Hantaan (HTN) virus and nephropathia epidemica (NE) virus were investigated using the indirect immunofluorescent antibody (IFA) technique. Sera were tested simultaneously against all three antigens, prepared as virus-infected E6 cells. The resulting antibody profiles were sufficiently distinct to permit discrimination between these viruses by IFA. In addition: | | |
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| | 1. | Sera from four American mammalogists and sera from meadow voles trapped in the United States reacted to highest titer against Prospect Hill virus and 2- to 4-fold and 4- to 8-fold, respectively, less to NE and HTN viruses. | | 2. | Sera from patients with classical, severe hemorrhagic fever with renal syndrome (HFRS) in Korea, Far Eastern USSR, and the People's Republic of China reacted to highest titer to HTN virus and 64- to 128-fold and 128- to 256-fold, respectively, lower to Prospect Hill virus and NE virus. | | 3. | Sera from the majority of patients with HFRS in Scandinavia, European USSR (Tula and Bashkiria), Hungary, and Yugoslavia reacted to highest titer to NE virus and 2-fold and 4-fold, respectively, lower to Prospect Hill virus and HTN virus. | | 4. | Six monoclonal antibodies produced to HTN virus were used to examine antigenic relationships by IFA between HTN, Prospect Hill and NE viruses. All six monoclonal antibodies reacted with HTN virus, but none reacted with Prospect Hill or NE viruses [2] . |
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