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  Russian Spring Summer Encephalitis   RSSEV

Arbovirus

Yes

4

Results of SALS surveys and information from the Catalogue.
Other Information
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B
Section VI - Biologic Characteristics

Blood (M)(LV), cerebro spinal fluid (M), CNS (M)(LV), heart (M) (LV), lung (M), liver spleen (M)(LV), kidney (M), nasopharyngeal (M), urine (M), lymph node (M)

Newborn and weanling mice, chick embryos, and guinea pigs; and primary avian cell cultures
 
 
Cell system (a) Virus passage history (b) Evidence of Infection
CPE PLAQUES Growth Without CPE
Day (c) Extent (d) Titer TCD50/ml (e) Day (c) Size (f) Titer PFU/ml (e) +/- (g)


CPE generally is irregular.
Plaques obtained by use of special media in chick or pig embryo cultures.
Multiplication of virus in chick embryo or pig embryo cell cultures obtained to titers 7-8 dex per ml. (titered as mouse infective doses).
Phenomenon of interference with CPE viruses can be used for titration of TBE-RSSE antibodies.
 
 
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