| | | | | PRNT antibody titers of rat immune sera to: |
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| Viruses | Nantaan | Seoul | Puumala | Prospect Hill | Belgrade |
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| | PRNT: Plaque-reduction neutralization test | | | | Nantaan | 1024 | <32 | <32 | <32 | <32 | | Seoul | <32 | 1024 | <32 | ND | <32 | | Puumala | <32 | <32 | 1024 | <32 | <32 | | Prospect Hill | <32 | <32 | <32 | 512 | <32 | | Belgrade | <32 | <32 | <32 | <32 | 2048 |
| | Titers are expressed as the reciprocal of the highest serum dilution producing 80% reduction in the number of virus plaques, as enumerated by neutral red or immunoperoxidase staining. | | Polymerase chain reaction. Using Hantaan virus M segment-specific (HanG2-I, 5' -TGGGCTGCAAGTGCATCAGAG -3'; HanG2-2, 5'-ACTGCTGTACAGCCTGTGCC-3' ) and Hantavirus genus-specific M segment (HG2F1, 5'-TGGGCTGCAAGTGC-3' ; hg2r1, 5'-CAACCCCAGCTAGTTTCA-3' ) oligonucleotide promer pairs, we were able to enzymatically amplify the 411-bp and 365-bp hantavirus sequence, respectively, in RNA extracted from Belgrade virus- infected Vero E6 calls. Restriction analysis if the enzymatically amplified, 411-bp product of Belgrade virus indicated that it is genetically distinct from Hantaan virus and from all known hantaviruses. | | |
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