| | | | | Antigen of Registered Virus |
|---|
| | HI [1] | CF [2] | NT [3] |
|---|
|
| | Immune Sera | Ht/Ho | Ratio | Ht/Ho | Ratio | Dex Prot. | | Buttonwillow | 320 | 1 | 128 | 1 | 3.5 | | California | 0/320 | 0 | 0/32 | 0 | 0.4 | | Tahyna | | | | | 0.6 | | Trivittatus | | | 0/128 | 0 | 0 | | BUT [4] | 5120 | 1 | 256(512) | 1 | | | Ingwavuma [4] | 160/5120 | 1/32 | 16/512 | 1/32 | | | Manzanilla [4] | | | 8/512 | 1/64 | | | Oropouche [4] | | | 16/256 | 1/16 | |
| | | | Immune Serum of Registered Virus |
|---|
| | HI [1] | CF [2] | NT [3] |
|---|
| Antigen | Ht/Ho | Ratio | Ht/Ho | Ratio | Dex Prot. |
|---|
|
| | Buttonwillow | 320 | 1 | 128 | 1 | 3.5 | | California | 40/320 | 1/8 | 0/128 | 0 | 2.0 | | Tahyna | 40/ND | | | | 0.8 | | Trivittatus | | | 0/128 | 0 | 1.2 | | BUT [4] | 5120 | 1 | 256(512) | 1 | | | Ingwavuma [4] | 640/5120 | 1/8 | 128/256 | 1/2 | | | Manzanilla [4] | 160/ND | | 16/512 | 1/32 | | | Oropouche [4] | | | 4/512 | 1/128 | |
| | Neutralization test in hamster kidney cell culture: No protection by the following immune sera: WEE, EEE, SLE, Powassan, Modoc, Rio Bravo, California, Cache Valley. Homologus immune serum protected against two dex virus. | | Screen CF tests [3] , Buttonwillow antigen and serum against following antigens and sera: Group A, WEE, EEE, Highland J, Uruma, Sindbis, Semliki, chikungunya; Group B, SLE, MVE. JBE, Powassan, Modoc, Ilheus, West Nile, Langat, dengues 1, 2, 3, Ntaya, Uganda S, yellow fever, Zika, Bussuquara, Kunjin; Group C: Marituba, Itaqui; California Group: Tahyna, Melao, Guaroa, trivittatus; Bunyamwera Group, Bunyamwera, Germiston, Wyeomia, Kairi, Ilesha, Chittoor, Cache Valley; Phlebotomus Fever Group, Naples, Sicilian; Bwamba, bluetongue, Junin, Anopheles A, Turlock, Colorado tick fever, Anopheles B, LCM, Theiler's virus, EMC. No crossing with above viruses was observed. Titer of Buttonwillow IMS 64, antigen 32. | | Immune rabbit serum--Dr. William McD. Hammon, University of Pittsburgh, found no inhibition of California or Tahyna antigens by Buttonwillow immune mouse serum [1] . | | SIRACA has antigenically classified Buttonwillow virus as a distinct virus type and placed it in the Manzanilla complex, one of a number of complexes comprising the Simbu serogroup [12] . | | |
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