| | | Antibodies prepared against more than 75 individual arboviruses and polyvalent grouping fluids representing 19 arbovirus serogroups were employed in preliminary virus identification attempts; in all, these polyclonal hyperimmune ascitic fluids are reactive with more than 200 individual arboviruses and other viruses. | | Initial complement-fixation tests were negative with all but the polyvalent grouping fluid prepared against Juona, Minatitlan, Gamboa, Alajuela, and Belem viruses. In a subsequent CF test with antibody to the individual viruses in this grouping fluid, AG83-1347 antigen reacted only with antibody to Jurona virus. When this antigen was tested by CF with antibody to the vesiculoviruses, Jurona, vesicular stomatitis Indianana, Cocal, Chandipura, Isfahan, Keuraliba, La Joya, Piry, and vesicular stomatitis New Jersey viruses, all but the antibody to Jurona virus reacted at 1:8 or less, with the Jurona antibody titering 1:32; homologous antibody titers were at least 256 and as high as >= 1024. | | AG83-1347 antigen (infected Vero cells) was tested by IFA with all recognized vesiculoviruses [2] . Antibodies to Jurona and Yug Bogdanovac viruses were the only ones reacting. By NT, Jurona virus is distinct from strain AG83-1347. | | Results of complement-fixation (CF), indirect flourescent antibody (IFA), and srum dilution-plaque reduction neutralization tests (NT) with strain AG83-1347 and Juroan virus [1] . | | | | TITER OF ANTIBODY TO : |
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| | | CF | IFA | NT |
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| Virus | Strain | AG83-1347 | JUR | AG83-1347 | JUR | AG83-1347 | JUR |
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| | | | | | | | | | | | | | | | | | Calchaqui | AG83-1347 | 256 | 32 | 20 | 80 | 40 | <10 | | Jurona | BeAr40578 | 16 | 512 | <10 | 1280 | <10 | >=10,240 | | | | | | | | |
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