• Component Description
• Eligible Sample
• Description of Laboratory Methodology
• Data Processing and Editing
• Laboratory Quality Assurance and Monitoring
• Analytic Notes
• References
• Codebook
  • SEQN - Respondent sequence number
  • WTSA2YR - Subsample A weights
  • URX14D - 2,5-dichlorophenol (ug/L) result
  • URD14DLC - 2,5-dichlorophenol comment
  • URXDCB - 2,4-dichlorophenol (ug/L) result
  • URDDCBLC - 2,4-dichlorophenol comment
  • URXUCR - Urinary creatinine (mg/dL)

National Health and Nutrition Examination Survey

2011-2012 Data Documentation, Codebook, and Frequencies

Pesticides - Environmental - Urine (PP_G)

Data File: PP_G.xpt

First Published: November 2013

Last Revised: October 2014

Component Description

Biomonitoring of environmental pesticides is used to determine their prevalence in humans and the relevance of human exposure in public health. The routes of human exposure to these phenolic compounds are industrial pollution, pesticide use, food consumption, or use of personal care products.

Chlorophenols have been used in the wood preservation industry, as intermediates in the production of pesticides, and as disinfectants or fungicides for industrial and indoor home use. The manufacture of other chlorinated aromatic compounds can produce chlorophenols as byproducts.

 

Eligible Sample

Examined participants aged 6 years and older from a one-third sample.

Description of Laboratory Methodology

A sensitive method for measuring two dichlorophenols, and several other phenols was developed in 2005 (Ye et al., 2005). The method uses on-line solid phase extraction (SPE) coupled to HPLC and tandem mass spectrometry (HPL/CMS/MS). With the use of isotopically labeled internal standards, the detection limits in 100 μL of urine are sufficient for measuring urinary levels of phenols in non-occupationally exposed subjects.

Refer to NHANES 2011-2012 Lab Methods for 2,4-dichlorophenol, 2,5-dichlorophenol for detailed description of the laboratory method used.

Data Processing and Editing

The data were reviewed. Incomplete data or improbable values were sent to the performing laboratory for confirmation.

Laboratory Quality Assurance and Monitoring

Urine specimens are processed, stored, and shipped to the Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention for analysis.

Detailed specimen collection and processing instructions are discussed in the NHANES Laboratory Procedures Manual (LPM). Vials are stored under appropriate frozen (–20°C) conditions until they are shipped to National Center for Environmental Health for testing.

Mobile Examination Centers (MECs)

Laboratory team performance is monitored using several techniques. NCHS and contract consultants use a structured quality assurance evaluation during unscheduled visits to evaluate both the quality of the laboratory work and the quality-control procedures. Each laboratory staff person is observed for equipment operation, specimen collection and preparation; testing procedures and constructive feedback are given to each staff. Formal retraining sessions are conducted annually to ensure that required skill levels were maintained.

The NHANES QA/QC protocols meet the 1988 Clinical Laboratory Improvement Amendments mandates. Detailed QA/QC instructions are discussed in the NHANES LPM.

Analytical Laboratories

NHANES uses several methods to monitor the quality of the analyses performed by the contract laboratories. In the MEC, these methods include performing blind split samples collected on “dry run” sessions. In addition, contract laboratories randomly perform repeat testing on 2.0% of all specimens.

NCHS developed and distributed a quality control protocol for all the contract laboratories which outlined the Westgard rules (Westgard et al, 1981) used when running NHANES specimens. Progress reports containing any problems encountered during shipping or receipt of specimens, summary statistics for each control pool, QC graphs, instrument calibration, reagents, and any special considerations are submitted to NCHS quarterly. The reports are reviewed for trends or shifts in the data. The laboratories are required to explain any identified areas of concern.

All QC procedures recommended by the manufacturers were followed. Reported results for all assays meet the Division of Laboratory Sciences’ quality control and quality assurance performance criteria for accuracy and precision, similar to the Westgard rules (Westgard et al, 1981).

Analytic Notes

Refer to the 2011-2012 Laboratory Data Overview for general information on NHANES laboratory data.

Subsample Weights

Urinary environmental pesticides were measured in a one third subsample of persons 6 years and older. Special sample weights are required to analyze these data properly. Specific sample weights for this subsample are included in this data file and should be used when analyzing these data.

Variance Estimation

The analysis of NHANES laboratory data must be conducted with the key survey design and basic demographic variables. The NHANES Demographic Data File contains demographic and sample design variables. The recommended procedure for variance estimation requires use of stratum and PSU variables (SDMVSTRA and SDMVPSU, respectively) in the demographic data file.

Links to NHANES Data Files

This laboratory data file can be linked to the other NHANES data files using the unique survey participant identifier SEQN.

Detection Limits

The detection limits were constant for all of the analytes in the data set. Two variables are provided for each of these analytes. The variable named URD___LC indicates whether the result was below the limit of detection. There are two values: “0” and “1”. “0” means that the result was at or above the limit of detection. “1” indicates that the result was below the limit of detection.

The other variable named URX___ provides the analytic result for that analyte. For analytes with analytic results below the lower limit of detection (i.e., URD___LC=1), an imputed fill value was placed in the analyte results field. This value is the lower limit of detection divided by square root of 2 (LLOD/sqrt(2)). 

The lower limit of detection (LLOD, in ug/L) for the environmental pesticides is:

Analyte	Item ID	LLOD
Urinary 2,4-dichlorophenol (ng/ml)	URXDCB	0.2
Urinary 2,5-dichlorophenol (ng/ml)	URX14D	0.2

Please refer to the NHANES Analytic Guidelines and the on-line NHANES Tutorial for further details on the use of sample weights and other analytic issues.

References

• Westgard JO, Barry PL, Hunt MR, Groth T. A multi-rule Shewhart chart for quality control in clinical chemistry. Clin Chem. 1981 Mar;27(3):493-501.

• Ye X, Kuklenyik Z, Needham LL, Calafat AM. Automated on-line column-switching HPLC-MS/MS method with peak focusing for the determination of nine environmental phenols in urine. Anal Chem. 2005 Aug 15;77(16):5407-13.

Codebook and Frequencies