Table of Contents

Component Description

Among participants aged 6-49 years in NHANES between 1999 and 2004 who were cytomegalovirus (CMV) IgG positive, had stored urine samples available, tested and found with urinary CMV shedding and sufficient DNA for genotyping.

Eligible Sample

Surplus urine from individuals aged 6 to 49 years who participated in NHANES years 1999 to 2004 and with urinary shedding (N=537).

Description of Laboratory Methodology

DNA Extraction: DNA was extracted from urine specimens by treatment with the QIAamp MinElute Media kit (Qiagen, Valencia, CA) then processed with the Qiacube automated extractor (Qiagen, Valencia, CA), both following manufacturer’s instructions.

PCR: The detection of CMV DNA was performed with polymerase chain reaction (PCR) targeting the envelope glycoprotein gB and gH genes by Vries et al., and using the MX 3005P Real-time PCR System (Agilent Technologies, New Castle, DE).

Laboratory Quality Assurance and Monitoring

Commercial reagents were used for all CMV detection. All QA/QC procedures recommended by the manufacturer were followed. The laboratory follows guidelines established by CDC Quality Management Systems (QMS) to ensure overall quality and consistency in diagnostic testing.

Data Processing and Editing

Data were submitted after all the testing on CMV genotyping was complete. The data were not edited.

Data Access: All data are publicly available.

Analytic Notes

There are six variables:

gB1: CMV gB1 variant (1=Positive, 2=Negative, 3=Insufficient DNA)

gB2: CMV gB2 variant (1=Positive, 2=Negative, 3=Insufficient DNA)

gB3: CMV gB3 variant (1=Positive, 2=Negative, 3=Insufficient DNA)

gB4: CMV gB4 variant (1=Positive, 2=Negative, 3=Insufficient DNA)

gH1: CMV gH1 variant (1=Positive, 2=Negative, 3=Insufficient DNA)

gH2: CMV gH2 variant (1=Positive, 2=Negative, 3=Insufficient DNA)

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 6 YEARS - 49 YEARS

SSgB1 - CMV gB1 variant

Variable Name:
SSgB1
SAS Label:
CMV gB1 variant
English Text:
CMV gB1 variant
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 Positive 105 105
2 Negative 183 288
3 Insufficient DNA 249 537
. Missing 0 537

SSgB2 - CMV gB2 variant

Variable Name:
SSgB2
SAS Label:
CMV gB2 variant
English Text:
CMV gB2 variant
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 Positive 57 57
2 Negative 231 288
3 Insufficient DNA 249 537
. Missing 0 537

SSgB3 - CMV gB3 variant

Variable Name:
SSgB3
SAS Label:
CMV gB3 variant
English Text:
CMV gB3 variant
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 Positive 43 43
2 Negative 245 288
3 Insufficient DNA 249 537
. Missing 0 537

SSgB4 - CMV gB4 variant

Variable Name:
SSgB4
SAS Label:
CMV gB4 variant
English Text:
CMV gB4 variant
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 Positive 28 28
2 Negative 260 288
3 Insufficient DNA 249 537
. Missing 0 537

SSgH1 - CMV gH1 variant

Variable Name:
SSgH1
SAS Label:
CMV gH1 variant
English Text:
CMV gH1 variant
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 Positive 121 121
2 Negative 167 288
3 Insufficient DNA 249 537
. Missing 0 537

SSgH2 - CMV gH2 variant

Variable Name:
SSgH2
SAS Label:
CMV gH2 variant
English Text:
CMV gH2 variant
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 Positive 148 148
2 Negative 140 288
3 Insufficient DNA 249 537
. Missing 0 537