National Health and Nutrition Examination Survey

1999-2000 Data Documentation, Codebook, and Frequencies

Mumps Antibody - Serum (Surplus) (SSMUMP_A)

Data File: SSMUMP_A.xpt

First Published: April 2008

Last Revised: April 2022

Note: The NHANES Biospecimen Program processes were reevaluated in 2021 and 2022 to monitor quality control after a procedural error was identified. This error did not pose any risk of participant disclosure. Addressing this error resulted in the removal of some records from various stored biospecimen data files between 1999 and 2018 that did not meet program standards. After a comprehensive review of all stored specimen datasets, this data file was modified to remove records (10-<15% of records) that were initially included in error. No data values were altered. However, survey weights were newly created. For each analyte included in this data file, it was determined that overall and for stratified sex, age, and race/Hispanic origin groups, the updated file using the new sample weights resulted in an estimate within the 95% confidence limit calculated using the original file and sample weights. However, not all possible analyses were performed. For any queries related to this dataset please email the Biospecimen Program at serumplasmaurine@cdc.gov.

Component Description

Mumps IgG antibody testing of stored serum specimens from NHANES (1999-2000) was conducted to estimate the seroprevalence of mumps antibody in the US population in 1999-2000.

Eligible Sample

Participants aged 6-49 years from of NHANES 1999-2000 with stored sera.

Description of Laboratory Methodology

Mumps antibody testing was performed using enzyme-linked immunosorbent assays (ELISAs) to detect mumps IgG (Wampole Laboratories, Princeton, NJ).

Specimens were tested according to manufacture directions.

Index values were calculated for each specimen by dividing the OD value by the cutoff OD.

Interpretation of Index Value of OD Ratio
Interpretation	Index Value of OD Ratio
Negative Specimens	≤0.900
Equivocal Specimens	0.901-1.099
Positive Specimens	≥1.100

Data Processing and Editing

Data Access: All data are publicly available.

Laboratory Quality Assurance and Monitoring

Serum samples were tested using Wampole Mumps IgG Test (Ref. # 425901CE). These were tested on two lots, the first lot used 19 kits and second lot used 57 kits.

Samples that initially tested Indeterminate or negative were repeated. If a discrepancy (difference in result and a change in ISR of > 0.5) was found the sample was repeated one more time. The previous result that matched the final test was the one accepted.

Assay reproducibility was assessed by random selection of 10% of the total number of serum specimens tested and repeating the ELISA assay.

Analytic Notes

The specimen index ratio (ISR) should be linked to the sequence number for all data analyses.

Subsample Weights

Sample weights are required to analyze these data properly. Specific sample weights for this subsample are included in this data file and should be used when analyzing these data. When observations were removed from this data file, new sample weight were created and added to this data file (WTSSMC2Y, WTSSMC4Y). Please refer to the NHANES Analytic Guidelines and the on-line NHANES Tutorial for further details on the use of sample weights and other analytic issues.

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:: SEQN
SAS Label:: Respondent sequence number
English Text:: Respondent sequence number.
Target:: Both males and females 6 YEARS - 49 YEARS

WTSSMC2Y - SSMUMP and SSCMV 2 year weights

Variable Name:: WTSSMC2Y
SAS Label:: SSMUMP and SSCMV 2 year weights
English Text:: Surplus specimen SSMUMP and SSCMV 2 year weights
Target:: Both males and females 6 YEARS - 49 YEARS

Code or Value	Value Description	Count	Cumulative
1250.36054 to 298307.64146	Range of Values	4136	4136
0	No lab specimen	3	4139
.	Missing	0	4139

WTSSMC4Y - SSMUMP and SSCMV 4 year weights 99-02

Variable Name:: WTSSMC4Y
SAS Label:: SSMUMP and SSCMV 4 year weights 99-02
English Text:: Surplus specimen SSMUMP and SSCMV 4 year weights 99-02
Target:: Both males and females 6 YEARS - 49 YEARS

Code or Value	Value Description	Count	Cumulative
769.842844 to 119080.51994	Range of Values	4136	4136
0	No lab specimen	3	4139
.	Missing	0	4139

SSISR - Specimen Index ratio

Variable Name:: SSISR
SAS Label:: Specimen Index ratio
English Text:: Specimen Index ratio
Target:: Both males and females 6 YEARS - 49 YEARS

Code or Value	Value Description	Count	Cumulative	Skip to Item
0.026 to 8.604	Range of Values	4132	4132
.	Missing	7	4139

SSMUMPS - Antibody to mumps

Variable Name:: SSMUMPS
SAS Label:: Antibody to mumps
English Text:: Antibody to mumps
Target:: Both males and females 6 YEARS - 49 YEARS

Code or Value	Value Description	Count	Cumulative
1	Positive	3770	3770
2	Negative	212	3982
3	Indeterminate	150	4132
9	Quantity not sufficient	0	4132
.	Missing	7	4139

Table of Contents