Table of Contents

Component Description

Among participants aged 6-49 years in NHANES between 1999 and 2004 who had stored urine samples available and were cytomegalovirus (CMV) IgG positive, urine specimens were tested with real time PCR to detect CMV.

Eligible Sample

Surplus urine from individuals aged 6 to 49 years who participated in NHANES years 1999 to 2004 and were CMV IgG+.

Description of Laboratory Methodology

DNA Extraction: DNA was extracted from urine specimens by treatment with the QIAamp MinElute Media kit (Qiagen, Valencia, CA) then processed with the Qiacube automated extractor (Qiagen, Valencia, CA), both following manufacturer’s instructions.

PCR: The detection of CMV DNA was performed with polymerase chain reaction (PCR) targeting the viral immediate early 2 (IE-2) region as described by Bopanna et al., and using the MX 3005P Real-time PCR System (Agilent Technologies, New Castle, DE).

Laboratory Quality Assurance and Monitoring

Commercial reagents were used for all CMV detection. All QA/QC procedures recommended by the manufacturer were followed. The laboratory follows guidelines established by CDC Quality Management Systems (QMS) to ensure overall quality and consistency in diagnostic testing.

Data Processing and Editing

Data were submitted after all the testing on CMV detection was complete. The data were not edited.

Data Access: All data are publicly available.

Analytic Notes

There are two variables:

Shedding: urinary CMV shedding results (1=Yes, 2=No, 3=Equivocal)

VirLoad: CMV urinary viral load Measured in copies/mL. Continuous variable - Values range from 68 to 707687.

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 6 YEARS - 49 YEARS

SSUCYSH - Cytomegalovirus shedding

Variable Name:
SSUCYSH
SAS Label:
Cytomegalovirus shedding
English Text:
Cytomegalovirus shedding
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 Positive 537 537
2 Negative 6291 6828
3 Equivocal 31 6859
. Missing 0 6859

SSUCTVL - Cytomegalovirus viral load Copies/mL

Variable Name:
SSUCTVL
SAS Label:
Cytomegalovirus viral load Copies/mL
English Text:
Cytomegalovirus viral load Copies/mL
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
68 to 707687 Range of Values 537 537
. Missing 6322 6859