Sera from consenting children age 6-13 years examined in NHANES 1999-2002 were tested for antibodies to Herpes Simplex Virus Type-1 (HSV-1) using a solid-phase enzymatic immunodot assay. Purified glycoprotein specific for HSV-1 (gG-1) was used as antigen. All results in this data release are reported as positive or negative or indeterminate.
All participants 6-13 years from NHANES 1999-2002 who gave consent for further testing using stored sera (n = 3,115).
Although extensive antigenic cross-reactivity exists between the two viral types of herpes, a viral glycoprotein specific for herpes simplex virus type 2 (HSV-2) (designated gG-2), and a glycoprotein specific for herpes simplex virus type 1 (HSV-1) (designated gG-1) have been identified. Monoclonal antibodies and affinity chromatography have been used to purify these glycoproteins and thus provide antigens for type-specific herpes serologic assays. Solid-phase enzymatic immunodot assays are used to detect antibodies reactive to these antigens. The purified glycoprotein gG-1 is adsorbed to the center of a nitrocellulose disk. The rest of the disk surface is coated with bovine serum albumin (BSA) to prevent further nonspecific protein adsorption. Incubation of test serum with the disk allows specific antibodies, if present, to bind to the immobilized antigen. After extensive washing to remove non-reactive antibodies, the bound antibodies are detected by sequential treatment with peroxidase-conjugated goat-anti-human IgG and the enzyme substrate (H2O2 with chromogen 4-chloro-1-naphthol). A positive reaction is demonstrated by the appearance of a blue dot at the center of the disk. Serum reactive to an immunodot charged with gG-1 indicates previous and probable latent HSV-1 infection.
Data was received after all the antibody testing was complete. The data were not edited.
Data Access: All data are publicly available.
Assay performance was monitored throughout the testing period by testing positive and negative controls derived from reference sera. gG-1 immunodot results were run in duplicate and 2% were randomly repeated.
SSHSVGG1 (HSV-1 antibody)
Please refer to the Analytic Guidelines for further details on the use of sample weights and other analytic issues.
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