Table of Contents

Component Description

Within the 2003-2004 National Health and Nutrition Examination Survey (NHANES) Serum Anti-Müllerian Hormone (AMH) and inhibin B concentrations among men were measured to evaluate variation by age and race/ethnicity. We also evaluate correlations with previously measured sex-steroid hormone measurements.

Eligible Sample

A sample of male participants across 9 age groups from stored NHANES 2003-2004 sera. Ages 12+. No weights are provided with this file.

Description of Laboratory Methodology

The Beckman Coulter Gen II AMH ELISA was used to measure AMH levels in the serum samples. The assay is used clinically in Europe and for research in the US and has excellent intra- and inter-plate reproducibility as reported in the literature (Wallace et al., 2011).

Immunoreactive inhibin B levels were measured in serum using ELISA. Samples will be pretreated with sodium dodecyl-sulfate, heat treated to 100°C, and exposed to hydrogen peroxide to enhance the specificity and sensitivity of the ELISA. The assay is based on specific antibody capture of the inhibin α and β subunits. Alkaline phosphatase is conjugated to the α subunit, and color intensity is determined by photometric analysis at an absorbance of 490 nm. The assay has a sensitivity of <15 pg/mL and is highly specific, with a <1% cross-reaction with inhibin A (Kolb et al., 2000).

The geometric mean values are reported.

Data Processing and Editing

Data was compiled after serum measurements were completed. The data were not edited.

Data Access: All data are publicly available.

Laboratory Quality Assurance and Monitoring

All QC procedures were followed according to established laboratory Special Operation Procedures.

Analytic Notes

There are 2 variables
AMH in ng/mL: geometric mean = 6.46 ng/mL range (0.002-11.23)
InhibinB in pg/mL: geometric mean = 117.36 pg/mL range (246.22-505.22)

Missing values were either not measurable due to insufficient quality or below the limit of detection.

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Males only 12 YEARS - 150 YEARS

SSAMH - Anti-Mullerian Hormone (ng/ml)

Variable Name:
SSAMH
SAS Label:
Anti-Mullerian Hormone (ng/ml)
English Text:
Anti-Mullerian Hormone (ng/ml)
Target:
Males only 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.031 to 246.217 Range of Values 505 505
. Missing 8 513

SSINHBIN - Inhibin B (pg/ml)

Variable Name:
SSINHBIN
SAS Label:
Inhibin B (pg/ml)
English Text:
Inhibin B (pg/ml)
Target:
Males only 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
11.926 to 425.982 Range of Values 495 495
. Missing 18 513