Table of Contents

Component Description

Antibodies to poliovirus serotypes 1, 2, and 3 assayed from stored serum samples from NHANES (2009-10)

Eligible Sample

All participants aged 6-49 years with available specimen and consent.

Description of Laboratory Methodology

Samples were tested in triplicate using a standard microneutralization assay for antibodies to poliovirus types 1, 2, and 3 according to established protocols at the Global Polio Specialized Laboratory, Centers for Disease Control and Prevention (CDC)[1,2]. Briefly, 80-100 CCID50 of each poliovirus serotype and two-fold serial dilutions of serum were combined and pre-incubated at 35°C for 3 hours before addition of HEp-2(C) cells. After incubation for 5 days at 35°C and 5% CO2, plates were stained with crystal violet and cell viability measured by optical density in a spectrophotometer. Each specimen was run in triplicate, with parallel specimens from one study subject tested in the same assay run, and the neutralization titers estimated by the Spearman-Kärber method [3] and reported as the reciprocal of the calculated 50% endpoint. A serum sample was considered positive if antibodies were present at ≥1:8 dilution. Specimens with antibody titers <1:8 were considered seronegative and specimens with titers >1:8 were considered seropositive.(1).

Data Processing and Editing

All data will be made publicly available.

Laboratory Quality Assurance and Monitoring

Each run contained multiple replicates of a reference antiserum pool starting at a 1:32 dilution to monitor performance variation.

Analytic Notes

All subjects will be used; therefore NHANES weights will be used for analyzing by age, sex, race/ethnicity, birthplace, and serum poliovirus antibody strata.

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 6 YEARS - 49 YEARS

SSPV1 - Antibody against wild type polio virus 1

Variable Name:
SSPV1
SAS Label:
Antibody against wild type polio virus 1
English Text:
Antibody against wild type polio virus 1
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
2.5 to 10.5 Range of Values 4806 4806
. Missing 0 4806

SSPV2 - Antibody against wild type polio virus 2

Variable Name:
SSPV2
SAS Label:
Antibody against wild type polio virus 2
English Text:
Antibody against wild type polio virus 2
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
2.5 to 10.5 Range of Values 4806 4806
. Missing 0 4806

SSPV3 - Antibody against wild type polio virus 3

Variable Name:
SSPV3
SAS Label:
Antibody against wild type polio virus 3
English Text:
Antibody against wild type polio virus 3
Target:
Both males and females 6 YEARS - 49 YEARS
Code or Value Value Description Count Cumulative Skip to Item
2.5 to 10.5 Range of Values 4806 4806
. Missing 0 4806