Table of Contents

Component Description

This data will establish baseline data to assist in disease diagnosis and the treatment and prevention of disease such as, Polycystic Ovary Syndrome (PCOS), androgen deficiency, cancer, and hormone imbalances in children. An estimated 5 to 7 million women in the United States (U.S) suffer with the effects of PCOS and PCOS can occur in girls as young as 11 years old. PCOS is the most common hormonal disorder among women of reproductive age and is the leading cause of infertility. The prevalence of symptomatic androgen deficiency in men between 30 and 79 years of age is estimated to be 5.6%. This increases substantially with age, effecting approximately 4 to 5 million men in the U.S. The aging of the U.S. male population will cause a large increase in the burden of symptomatic androgen deficiency.

Measurement of testosterone (T measurements) based on current research could provide insight into the diagnosis of several diseases and disorders. Examples include the detection of hormone-secreting tumors found in the breast and prostate. In these cases, T measurements may aid in the diagnosis of breast cancer18 and in addition, because high testosterone levels have been associated with increased prostate cancer risk19, may aid in the diagnosis of prostate cancer. Additionally, it has been observed that changes in hormone levels in population studies were associated with several diseases including coronary artery disease20 and diabetes in men20. As a result, T measurements could be used to report possible risk factors.

Eligible Sample

Participants aged 6 years and older, who do not meet any of the exclusion criteria, are eligible.

Description of Laboratory Methodology

This is a validated isotope dilution liquid chromatography tandem mass spectrometry (ID-LC-MS/MS) method for routine quantitation of serum total testosterone based on the National Institute for Standards and Technology’s (NIST) reference method. This method was optimized for higher sample throughput.

This method employs LLE extractions of serum to isolate the steroid. Stable Isotope-labeled testosterone is used as an internal standard to correct for sample recovery during the sample preparation process. Assay imprecision is 5% and the method is traceability to an established higher-order testosterone reference method (NIST method).

Data Processing and Editing

Serum specimens are processed, stored, and shipped to the Division of Environmental Health Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention for analysis.

Detailed specimen collection and processing instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM). Vials are stored under appropriate frozen (–20°C) conditions until they are shipped to National Center for Environmental Health for testing.

This file contains no top coding.

Laboratory Quality Assurance and Monitoring

The NHANES quality control and quality assurance protocols (QA/QC) meet the 1988 Clinical Laboratory Improvement Act mandates. Detailed quality control and quality assurance instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM). Read the LABDOC file for detailed QA/QC protocols.

Analytic Notes

Refer to the 2011-2012 Laboratory Data Overview for general information on NHANES laboratory data.

The analysis of NHANES 2011-2012 laboratory data must be conducted using the appropriate survey design and demographic variables. The NHANES 2011-2012 Demographics File contains demographic data, health indicators, and other related information collected during household interviews as well as the sample weight variables. The Fasting Questionnaire File includes auxiliary information such as fasting status, the time of venipuncture, and the conditions precluding venipuncture. The demographics and fasting questionnaire files may be linked to the laboratory data file using the unique survey participant identifier (i.e., SEQN).

Please refer to the NHANES Analytic Guidelines and the on-line NHANES Tutorial for further details on the use of sample weights and other analytic issues. The Analytic Guidelines are available on the NHANES website.

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number
Target:
Both males and females 6 YEARS - 150 YEARS

LBXTST - Testosterone total (ng/dL)

Variable Name:
LBXTST
SAS Label:
Testosterone total (ng/dL)
English Text:
Testosterone total (ng/dL)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.25 to 2543.99 Range of Values 6826 6826
. Missing 995 7821