Table of Contents

Component Description

Flame retardants (FRs) are either additive or reactive ingredients applied to household and consumer products to reduce the products flammability, and to meet state and federal fire safety standards and regulations. Until recently, the dominant class of FR additives used for household products was polybrominated diphenyl ethers (PBDEs), which are persistent and can accumulate in the environment (de Wit, 2002; Law 2006, Stapleton 2012). Flame retardant formulations containing chlorinated and non-chlorinated organophosphates and products such as Firemaster 550 (FM550) have entered the consumers’ market worldwide as PBDEs have been phased-out in many countries (van der Veen, 2012). We recently developed a method to measure diphenyl phosphate, bis(1,3-dichloro-2-propyl) phosphate), bis(1-chloro-2-propyl) phosphate, bis(2-chloroethyl) phosphate, di-p-cresyl phosphate, di-o-cresyl phosphate, dibutyl phosphate, dibenzyl phosphate, and 2,3,4,5-tetrabromobenzoic acid . These compounds are urinary metabolites of triphenyl phosphate, tris(1,3-dichloro-2-propyl) phosphate, tris(1-chloro-2-propyl) phosphate, tris(2-chloroethyl) phosphate, tri-cresyl phosphates, tributyl phosphate, tribenzyl phosphate, and 2-ethylhexyl-2,3,4,5-tetrabromobenzoate, respectively, and can be used as exposure biomarkers for these compounds.

Eligible Sample

A random 1/3 sample of participants aged 6+ years from NHANES 2013-2014 with stored urine.

Description of Laboratory Methodology

The method uses 0.4 mL urine and is based on enzymatic hydrolysis of urinary conjugates of the target analytes, automated off-line solid phase extraction, reversed phase high-performance liquid chromatography separation, and isotope dilution-electrospray ionization tandem mass spectrometry detection (Jayatilaka 2017).

Laboratory Quality Assurance and Monitoring

The analytical measurements were conducted following strict quality control/quality assurance CLIA guidelines. Along with the study samples, each analytical run included high- and low-concentration quality control materials (QCs) and reagent blanks to assure the accuracy and reliability of the data. The concentrations of the high-concentration QCs and the low-concentration QCs, averaged to obtain one measurement of high-concentration QC and low-concentration QC for each run, were evaluated using standard statistical probability rules (Caudill et al. 2008).

Data Processing and Editing

Data were received after all analyses were complete. The data were not edited. Data Access: All data are publicly available.

Analytic Notes

Detection Limits

The detection limits were constant for all of the analytes in the data set. Two variables are provided for each analyte. The variable name ending in “L” (ex., SSDPHPL) indicates whether the result was below the limit of detection: the value “0” means that the result was at or above the limit of detection, “1” indicates that the result was below the limit of detection. For analytes with analytic results below the limit of detection (ex., SSDPHPL=1), an imputed fill value was placed in the analyte results field. This value is the limit of detection divided by square root of 2 (LOD/√2). The other variable prefixed SS (ex., SSDPHP) provides the analytic result for that analyte.

The limit of detection (LOD, in µg/L) for DPhP, BDCPP, BCPP, BCEtP, DpCP, DoCP, DBuP, DBzP and TBBA:

Variable Name SAS Label LLOD
SSDPHP Diphenyl phosphate (µg/L) 0.16
SSBDCPP Bis(1,3-dichloro-2-propyl) phosphate (µg/L) 0.11
SSBCPP Bis(1-chloro-2-propyl) phosphate (µg/L) 0.10
SSBCEP Bis(2-chloroethyl) phosphate (µg/L) 0.08
SSDPCP Di-p-cresyl phosphate (µg/L) 0.05
SSDOCP Di-o-cresyl phosphate (µg/L) 0.05
SSDBUP Dibutyl phosphate (µg/L) 0.05
SSDBZP Dibenzyl phosphate (µg/L) 0.05
SSTBBA 2,3,4,5-tetrabromobenzoic acid (µg/L) 0.05

Interferences:

Blanks in an analyte results field represent missing values in cases when the presence of interferences precluded obtaining a valid numeric result for a given analyte.

Subsample Weights

Sample weights are required to analyze these data properly. Specific sample weights for this subsample are included in this data file and should be used when analyzing these data. Previous versions of this data file included a sample weight variable (WTSC2YR) but when observations were removed from this data file, a new sample weight was created, and a new sample weight variable was added to this data file (WTSSCH2Y). Please refer to the NHANES Analytic Guidelines and the on-line NHANES Tutorial for further details on the use of sample weights and other analytic issues.

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 6 YEARS - 150 YEARS

WTSSCH2Y - Surplus specimen C 13-14 2 year weights

Variable Name:
WTSSCH2Y
SAS Label:
Surplus specimen C 13-14 2 year weights
English Text:
Surplus specimen 2 year weights C for 2013-2014
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
19943.069763 to 604985.17222 Range of Values 2317 2317
0 No lab specimen 0 2317
. Missing 0 2317

SSDPHP - Diphenyl phosphate (ug/L)

Variable Name:
SSDPHP
SAS Label:
Diphenyl phosphate (ug/L)
English Text:
Diphenyl phosphate (ug/L)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.11 to 193 Range of Values 2312 2312
. Missing 5 2317

SSDPHPL - Diphenyl phosphate cmt code

Variable Name:
SSDPHPL
SAS Label:
Diphenyl phosphate cmt code
English Text:
Diphenyl phosphate comment code
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 2116 2116
1 Below lower detection limit 196 2312
. Missing 5 2317

SSBDCPP - Bis(1,3-dichloro-2-propyl) phospht(ug/L)

Variable Name:
SSBDCPP
SAS Label:
Bis(1,3-dichloro-2-propyl) phospht(ug/L)
English Text:
Bis(1,3-dichloro-2-propyl) phosphate (ug/L)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.08 to 169 Range of Values 2299 2299
. Missing 18 2317

SSBDCPPL - Bis(1,3-dichloro-2-propyl) phosphate cmt

Variable Name:
SSBDCPPL
SAS Label:
Bis(1,3-dichloro-2-propyl) phosphate cmt
English Text:
Bis(1,3-dichloro-2-propyl) phosphate comment code
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 2127 2127
1 Below lower detection limit 172 2299
. Missing 18 2317

SSBCPP - Bis(1-chloro-2-propyl) phosphate (ug/L)

Variable Name:
SSBCPP
SAS Label:
Bis(1-chloro-2-propyl) phosphate (ug/L)
English Text:
Bis(1-chloro-2-propyl) phosphate (ug/L)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.07 to 14.6 Range of Values 2316 2316
. Missing 1 2317

SSBCPPL - Bis(1-chloro-2-propyl) phosphate cmt

Variable Name:
SSBCPPL
SAS Label:
Bis(1-chloro-2-propyl) phosphate cmt
English Text:
Bis(1-chloro-2-propyl) phosphate comment code
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 1400 1400
1 Below lower detection limit 916 2316
. Missing 1 2317

SSBCEP - Bis(2-chloroethyl) phosphate (ug/L)

Variable Name:
SSBCEP
SAS Label:
Bis(2-chloroethyl) phosphate (ug/L)
English Text:
Bis(2-chloroethyl) phosphate (ug/L)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.06 to 110 Range of Values 2303 2303
. Missing 14 2317

SSBCEPL - Bis(2-chloroethyl) phosphate cmt code

Variable Name:
SSBCEPL
SAS Label:
Bis(2-chloroethyl) phosphate cmt code
English Text:
Bis(2-chloroethyl) phosphate comment code
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 2052 2052
1 Below lower detection limit 251 2303
. Missing 14 2317

SSDPCP - Di-p-cresyl phosphate (ug/L)

Variable Name:
SSDPCP
SAS Label:
Di-p-cresyl phosphate (ug/L)
English Text:
Di-p-cresyl phosphate (ug/L)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.04 to 1.62 Range of Values 2316 2316
. Missing 1 2317

SSDPCPL - Di-p-cresyl phosphate cmt code

Variable Name:
SSDPCPL
SAS Label:
Di-p-cresyl phosphate cmt code
English Text:
Di-p-cresyl phosphate comment code
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 310 310
1 Below lower detection limit 2006 2316
. Missing 1 2317

SSDOCP - Di-o-cresyl phosphate (ug/L)

Variable Name:
SSDOCP
SAS Label:
Di-o-cresyl phosphate (ug/L)
English Text:
Di-o-cresyl phosphate (ug/L)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.04 to 1.1 Range of Values 2316 2316
. Missing 1 2317

SSDOCPL - Di-o-cresyl phosphate cmt code

Variable Name:
SSDOCPL
SAS Label:
Di-o-cresyl phosphate cmt code
English Text:
Di-o-cresyl phosphate comment code
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 1 1
1 Below lower detection limit 2315 2316
. Missing 1 2317

SSDBUP - Dibutyl phosphate (ug/L)

Variable Name:
SSDBUP
SAS Label:
Dibutyl phosphate (ug/L)
English Text:
Dibutyl phosphate (ug/L)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.04 to 14.3 Range of Values 2314 2314
. Missing 3 2317

SSDBUPL - Dibutyl phosphate cmt code

Variable Name:
SSDBUPL
SAS Label:
Dibutyl phosphate cmt code
English Text:
Dibutyl phosphate comment code
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 1863 1863
1 Below lower detection limit 451 2314
. Missing 3 2317

SSDBZP - Dibenzyl phosphate (ug/L)

Variable Name:
SSDBZP
SAS Label:
Dibenzyl phosphate (ug/L)
English Text:
Dibenzyl phosphate (ug/L)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.04 0.04 2316 2316
. Missing 1 2317

SSDBZPL - Dibenzyl phosphate cmt code

Variable Name:
SSDBZPL
SAS Label:
Dibenzyl phosphate cmt code
English Text:
Dibenzyl phosphate comment code
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 0 0
1 Below lower detection limit 2316 2316
. Missing 1 2317

SSTBBA - 2,3,4,5-tetrabromobenzoic acid (ug/L)

Variable Name:
SSTBBA
SAS Label:
2,3,4,5-tetrabromobenzoic acid (ug/L)
English Text:
2,3,4,5-tetrabromobenzoic acid (ug/L)
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.04 to 0.82 Range of Values 2316 2316
. Missing 1 2317

SSTBBAL - 2,3,4,5-tetrabromobenzoic acid cmt code

Variable Name:
SSTBBAL
SAS Label:
2,3,4,5-tetrabromobenzoic acid cmt code
English Text:
2,3,4,5-tetrabromobenzoic acid comment code
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 115 115
1 Below lower detection limit 2201 2316
. Missing 1 2317