• Component Description
• Eligible Sample
• Description of Laboratory Methodology
• Laboratory Quality Assurance and Monitoring
• Data Processing and Editing
• Analytic Notes
• References
• Codebook
  • SEQN - Respondent sequence number
  • WTSSGL2Y - Surplus specimen GLYP 15-16 2 yr weights
  • SSGLYP - Glyphosate (ng/mL)
  • SSGLYPL - Glyphosate comment code

National Health and Nutrition Examination Survey

2015-2016 Data Documentation, Codebook, and Frequencies

Glyphosate (GLYP) - Urine (Surplus) (SSGLYP_I)

Data File: SSGLYP_I.xpt

First Published: November 2022

Last Revised: NA

Component Description

Glyphosate is the most widely used herbicide in the United States in the agricultural sector and the second most used in the home and garden market sector (U.S. EPA, 2017). Its use has increased more than 200-fold since 1974 when Monsanto commercialized it under the trade name Roundup (Benbrook, 2016). In 2015, the International Agency for Research on Cancer determined that glyphosate is a “probable human carcinogen” (IARC, 2015). However, the European Food Safety Authority and the Joint Food and Agriculture Organization (FAO)/World Health Organization (WHO) Meeting on Pesticide Residues (EFSA, 2015; FAO/WHO, 2015) determined that glyphosate is unlikely to be a carcinogen. The U.S. EPA concluded that “available data and weight-of-evidence clearly do not support the descriptors “carcinogenic to humans,” “likely to be carcinogenic to humans,” or “inadequate information to assess carcinogenic potential” (U.S. EPA, 2017a). Controversy and concern that the rising use of glyphosate may have adverse human-health effects exist (Myers et. al., 2016). Yet, the extent of human exposure to glyphosate in the U.S. population is largely unknown. Data to better understand exposure to glyphosate are needed to increase our knowledge of its potential effects on health.

Eligible Sample

All examined participants aged 3 to 5 years and a one-third subsample of examined participants aged 6 years and older were eligible.

Description of Laboratory Methodology

This method used 200 microliters of urine and was based on 2D-on-line ion chromatography coupled with tandem mass spectrometry (IC- MS/MS) and isotope dilution quantification (Schutze et. al., 2021).

There were no changes to the lab method, lab equipment, or lab site for this component in the NHANES 2015-2016 cycle.

Laboratory Quality Assurance and Monitoring

The analytical measurements were conducted following strict quality control/quality assurance CLIA guidelines. Along with the study samples, each analytical run included high- and low-concentration quality control materials (QCMs) and reagent blanks to assure the accuracy and reliability of the data. The concentrations of the high-concentration QCMs and the low-concentration QCMs, averaged to obtain one measurement of high-concentration QCM and low-concentration QCM for each run, were evaluated using standard statistical probability rules (Caudill et. al., 2008).

Data Processing and Editing

Data were received after all analyses were complete. The data were not edited. The data were reviewed. Incomplete data or improbable values were sent to the performing laboratory for confirmation.

Analytic Notes

Refer to the 2015 – 2016 Laboratory Data Overview for general information on NHANES laboratory data.

There are over 800 laboratory tests performed on NHANES participants. However, not all participants provided biospecimens or enough volume for all the tests to be performed. Additionally, availability of specimens for surplus projects is lower than for other laboratory tests performed on NHANES participants. The specimen availability can also vary by age or other population characteristics. Analysts should evaluate the extent of missing data in the dataset related to the outcome of interest as well as any predictor variables used in the analyses to determine whether additional re-weighting for item non-response is necessary.

Please refer to the NHANES Analytic Guidelines and the on-line NHANES Tutorial for further details on the use of sample weights and other analytic issues.

Subsample Weights

The analytes included in this dataset were measured in all examined participants aged 3-5 years, and in a one-third subsample of participants 6 years and older. Special sample weights are required to analyze these data properly. Specific sample weights for this subsample, WTSSGL2Y, are included in this data file and should be used when analyzing these data. The sample weights created for this file used the examination sample weight, i.e., WTMEC2YR, as the base weight. The base weight was adjusted for additional nonresponse to these lab tests and re-poststratified to the population total using sex, age, and race/ethnicity. Participants who were part of the eligible population but who did not provide a urine specimen, or did not have sufficient volume of biospecimens, or who did not give consent for their specimens to be used for future research are included in the file, but they have a sample weight assigned “0” in their records.

Demographic and Other Related Variables

The analysis of NHANES laboratory data must be conducted using the appropriate survey design and demographic variables. The NHANES 2015-2016 Demographics File contains demographic data, health indicators, and other related information collected during household interviews as well as the sample design variables. The recommended procedure for variance estimation requires use of stratum and PSU variables (SDMVSTRA and SDMVPSU, respectively) in the demographic data file.

This laboratory data file can be linked to the other NHANES data files using the unique survey participant identifier (i.e., SEQN).

The variable URXUCR (urine creatinine) will not be reported in this file. URXUCR can be found in the data file titled “Albumin & Creatinine – Urine”.

Detection Limits

The detection limit was constant for all of the analytes in the data set. Two variables are provided for each of these analytes. The variable name ending in “L” (ex., SSGLYPL) indicates whether the result was below the limit of detection: the value “0” means that the result was at or above the limit of detection, “1” indicates that the result was below the limit of detection. The other variable (ex., SSGLYP) provides the analytic result for that analyte. For analytes with analytic results below the lower limit of detection (ex., SSGLYPL=1), an imputed fill value was placed in the analyte results field. This value is the lower limit of detection divided by square root of 2 (LLOD/sqrt[2]). The lower limit of detection (LLOD, in ng/mL) for GLYP:

Variable Name	Analyte Description	LLOD
SSGLYP	Glyphosate (ng/mL)	0.2

References

• Benbrook C.M. Trends in glyphosate herbicide use in the United States and globally. Environ. Sci. Eur. (2016) 28:3.
• Caudill S.P., Schleicher R.L., Pirkle J.L.. Multi-rule quality control for the age-related eye disease study. Statist. Med. (2008) 27(20): 4094-4106.
• EFSA. Conclusion on the peer review of the pesticide risk assessment of the active substance glyphosate. EFSA Journal. (2015) 13(11): 4302.
• FAO/WHO. Joint FAO/WHO Meeting on Pesticide Residues, Summary Report. (2016). Available from: https://www.fao.org/fileadmin/templates/agphome/documents/Pests_Pesticides/JMPR/2016_JMPR_Summary_Special.pdf.
• IARC (International Agency for Research on Cancer). Monographs, Volume 112: Some organophosphate insecticides and herbicides: tetrachlorvinphos, parathion, malathion, diazinon and glyphosate. IARC Monogr. Eval. Carcinog. Risk Chem. Hum. IARC Working Group. Lyon; 3–10 March 2015. (2015).
• Myers J.P., Antoniou M.N., Blumberg B., et al. Concerns over use of glyphosate-based herbicides and risks associated with exposures. Environ. Health. (2016) 15:19.
• Schütze A., Morales-Agudelo P., Vidal M., Calafat A.M., and Ospina M. Quantification of glyphosate and other organophosphorus compounds in human urine via ion chromatography isotope dilution tandem mass spectrometry. Chemosphere. (2021) 274(129427).
• U.S. EPA 2017. Pesticides Industry Sales and Usage 2008-2012 Estimates. (2017) Available from: https://www.epa.gov/sites/production/files/2017-01/documents/pesticides-industry-sales-usage-2016_0.pdf.
• U.S. EPA 2017a. Revised Glyphosate Issue Paper: Evaluation of Carcinogenic Potential. EPA’s Office of Pesticide Programs December 12, 2017. (2017) Available from: https://cfpub.epa.gov/si/si_public_file_download.cfm?p_download_id=534487.

Codebook and Frequencies