• Component Description
• Eligible Sample
• Description of Laboratory Methodology
• Laboratory Method Files
• Laboratory Quality Assurance and Monitoring
• Data Processing and Editing
• Analytic Notes
• References
• Codebook
  • SEQN - Respondent Sequence Number
  • WTFSM - Two Year Smoking Weights
  • URX1DC - N-acel-S-(1,2-dichlorovinl)-L-cys(ng/mL)
  • URD1DCLC - N-acel-S-(1,2-dichlorovinl)-L-cys comt
  • URX2DC - N-acel-S-(2,2-dichlorvinyl)-L-cys(ng/mL)
  • URD2DCLC - N-acel-S-(2,2-dichlorvinyl)-L-cys comt
  • URX2MH - 2-methylhippuric acid (ng/mL)
  • URD2MHLC - 2-methylhippuric acid comment code
  • URX34M - 3-methipurc acd & 4-methipurc acd(ng/mL)
  • URD34MLC - 3-methipurc acd & 4-methipurc acid comt
  • URXAAM - N-ace-S-(2-carbamoylethyl)-L-cys(ng/mL)
  • URDAAMLC - N-ace-S-(2-carbamoylethyl)-L-cys comt
  • URXAMC - N-ace-S-(N-methlcarbamoyl)-L-cys(ng/mL)
  • URDAMCLC - N-ace-S-(N-methlcarbamoyl)-L-cys comt
  • URXATC - 2-amnothiazolne-4-carbxylic acid(ng/mL)
  • URDATCLC - 2-amnothiazolne-4-carbxylic acid comt
  • URXBMA - N-acetyl-S-(benzyl)-L-cysteine(ng/mL)
  • URDBMALC - N-acetyl-S-(benzyl)-L-cysteine comt
  • URXBPM - N-acetyl-S-(n-propyl)-L-cysteine(ng/mL)
  • URDBPMLC - N-acetyl-S-(n-propyl)-L-cysteine comt
  • URXCEM - N-acetyl-S-(2-carbxyethyl)-L-cys(ng/mL)
  • URDCEMLC - N-acetyl-S-(2-carbxyethyl)-L-cys comt
  • URXCYHA - CYHA cysteine (ng/mL)
  • URDCYALC - CYHA cysteine comment code
  • URXCYM - N-acetyl-S-(2-cyanoethyl)-L-cyst(ng/mL)
  • URDCYMLC - N-acetyl-S-(2-cyanoethyl)-L-cyst comt
  • URXDHB - N-ace-S-(3,4-dihidxybutl)-L-cys(ng/mL)
  • URDDHBLC - N-ace-S-(3,4-dihidxybutl)-L-cys comt
  • URXDPM - N-ace-S-(dimethylphenyl)-L-cys(ng/mL)
  • URDDPMLC - N-ace-S-(dimethylphenyl)-L-cys comt
  • URXGAM - N-ac-S-(2-carbmo-2-hydxel)-L-cys(ng/mL)
  • URDGAMLC - N-ac-S-(2-carbmo-2-hydxel)-L-cys comt
  • URXHEM - N-ace-S-(2-hydroxyethyl)-L-cys(ng/mL)
  • URDHEMLC - N-ace-S-(2-hydroxyethyl)-L-cys comt
  • URXHP2 - N-ace-S-(2-hydroxypropyl)-L-cys(ng/mL)
  • URDHP2LC - N-ace-S-(2-hydroxypropyl)-L-cys comt
  • URXHPM - N-ace-S-(3-hydroxypropyl)-L-cys(ng/mL)
  • URDHPMLC - N-ace-S-(3-hydroxypropyl)-L-cys comt
  • URXIPM1 - IPM1 cysteine (ng/mL)
  • URDPM1LC - IPM1 cysteine comment code
  • URXIPM3 - IPM3 cysteine (ng/mL)
  • URDPM3LC - IPM3 cysteine comment code
  • URXMAD - Mandelic acid(ng/mL)
  • URDMADLC - Mandelic acid comment code
  • URXMB1 - N-A-S-(1-hydrxmet)-2-Prpn)-L-cys(ng/mL)
  • URDMB1LC - N-A-S-(1-hydrxmet)-2-Prpn)-L-cys comt
  • URXMB2 - N-Ac-S-(2-hydrxy-3-butenyl)-L-cys(ng/mL)
  • URDMB2LC - N-Ac-S-(2-hydrxy-3-butenyl)-L-cys comt
  • URXMB3 - N-A-S-(4-hydrxy-2-butenyl)-L-cys(ng/mL)
  • URDMB3LC - N-A-S-(4-hydrxy-2-butenyl)-L-cys comt
  • URXPHE - N-ace-S-(phenl-2-hydxyetl)-L-cys(ng/mL)
  • URDPHELC - N-ace-S-(phenl-2-hydxyetl)-L-cys comt
  • URXPHG - Phenylglyoxylic acid(ng/mL)
  • URDPHGLC - Phenylglyoxylic acid comment code
  • URXPMA - N-acetyl-S-(phenyl)-L-cysteine(ng/mL)
  • URDPMALC - N-acetyl-S-(phenyl)-L-cysteine comt
  • URXPMM - N-A-S-(3-hydrxprpl-1-metl)-L-cys(ng/mL)
  • URDPMMLC - N-A-S-(3-hydrxprpl-1-metl)-L-cys comt
  • URXTCV - N-acetyl-S-(trichlorovinyl)-L-cys(ng/mL)
  • URDTCVLC - N-acetyl-S-(trichlorovinyl)-L-cys comt

National Health and Nutrition Examination Survey

2015-2016 Data Documentation, Codebook, and Frequencies

Volatile Organic Compound (VOC) Metabolites - Urine - Special Sample (UVOCS_I)

Data File: UVOCS_I.xpt

First Published: November 2018

Last Revised: NA

Component Description

Exposure to volatile organic compounds (VOCs) is ubiquitous. Chronic exposure to extremely high levels of some VOCs can lead to cancer and neurocognitive dysfunction. Urinary metabolites of VOCs can be detectable in urine for a longer period of time than the parent VOCs can be detected in blood.

Nearly 200 air toxicants have been associated with adverse health effects in occupational studies or laboratory studies, but have not been monitored in general population groups. Information on levels of exposure to these compounds, as measured by their metabolite levels in urine, is essential to determine the need for regulatory mechanisms to reduce the levels of hazardous air pollutants to which the general population is exposed.

Eligible Sample

Examined participants aged 18 years and older from a one-third subsample were eligible. Additionally, to oversample adult smokers, those participants aged 18 years and older, not in the regular one-third subsample, who smoked at least 100 cigarettes in their entire lifetime (SMQ020=1) and now smoke cigarettes every day (SMQ040=1), were also included in this special subsample.

Description of Laboratory Methodology

This method is a quantitative procedure for the measurement of VOC metabolites in human urine using ultra performance liquid chromatography coupled with electrospray tandem mass spectrometry (UPLC-ESI/MSMS) as described by Alwis et al (2012). Chromatographic separation is achieved using an Acquity UPLC® HSS T3 (Part no. 186003540, 1.8 µm x 2.1 mm x 150 mm, Waters Inc.) column with 15 mM ammonium acetate and acetonitrile as the mobile phases. The eluent from the column is ionized using an electrospray interface to generate and transmit negative ions into the mass spectrometer. Comparison of relative response factors (ratio of native analyte to stable isotope labeled internal standard) with known standard concentrations yields individual analyte concentrations.

Refer to the Laboratory Method Files section for a detailed description of the laboratory methods used.

There were no changes to the lab method, lab equipment, or lab site for this component in the NHANES 2015-2016 cycle.

Laboratory Method Files

Urinary VOC Metabolites Laboratory Procedure Manual (November 2018)

Laboratory Quality Assurance and Monitoring

Urine specimens are processed, stored, and shipped to the Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta GA for analysis.

Detailed instructions on specimen collection and processing are discussed in the NHANES Laboratory Procedures Manual (LPM). Vials are stored under appropriate frozen (–20°C) conditions until they are shipped to National Center for Environmental Health for testing.

The NHANES QA/QC protocols meet the 1988 Clinical Laboratory Improvement Act mandates. Detailed QA/QC instructions are discussed in the NHANES LPM.

Mobile Examination Centers (MECs)

Laboratory team performance is monitored using several techniques. NCHS and contract consultants use a structured competency assessment evaluation during visits to evaluate both the quality of the laboratory work and the quality-control procedures. Each laboratory staff member is observed for equipment operation, specimen collection and preparation; testing procedures and constructive feedback are given to each staff member. Formal retraining sessions are conducted annually to ensure that required skill levels were maintained.

Analytical Laboratories

NHANES uses several methods to monitor the quality of the analyses performed by the contract laboratories. In the MEC, these methods include performing blind split samples collected during “dry run” sessions. In addition, contract laboratories randomly perform repeat testing on 2% of all specimens.

NCHS developed and distributed a quality control protocol for all CDC and contract laboratories, which outlined the use of Westgard rules (Westgard, et al. 1981) when running NHANES specimens. Progress reports containing any problems encountered during shipping or receipt of specimens, summary statistics for each control pool, QC graphs, instrument calibration, reagents, and any special considerations are submitted to NCHS quarterly. The reports are reviewed for trends or shifts in the data. The laboratories are required to explain any identified areas of concern.

All QC procedures recommended by the manufacturers were followed. Reported results for all assays meet the Division of Laboratory Sciences’ quality control and quality assurance performance criteria for accuracy and precision, similar to the Westgard rules (Caudill et al., 2008).

Data Processing and Editing

The data were reviewed. Incomplete data or improbable values were sent to the performing laboratory for confirmation.

Analytic Notes

Refer to the 2015-2016 Laboratory Data Overview for general information on NHANES laboratory data.

Subsample Weights

VOC metabolites in urine were measured in a special smoking subsample of participants aged 18 years and older. Special sample weights are required to analyze these data properly. Specific sample weights for this subsample are included in this data file and should be used when analyzing these data.

Demographic and Other Related Variables

The analysis of NHANES laboratory data must be conducted using the appropriate survey design and demographic variables. The NHANES 2015-2016 Demographics File contains demographic data, health indicators, and other related information collected during household interviews as well as the sample design variables. The recommended procedure for variance estimation requires use of stratum and PSU variables (SDMVSTRA and SDMVPSU, respectively) in the demographic data file.

This laboratory data file can be linked to the other NHANES data files using the unique survey participant identifier (i.e., SEQN).

Detection limits

The detection limits were constant for the analytes in the data set. Two variables are provided for each of these analytes. The variable named ending in “LC” (ex., URDAAMLC) indicates whether the result was below the limit of detection: the value “0” means that the result was at or above the limit of detection, “1” indicates that the result was below the limit of detection. For analytes with analytic results below the lower limit of detection (ex., URDAAMLC=1), an imputed fill value was placed in the analyte results field. This value is the lower limit of detection divided by the square root of 2 (LLOD/sqrt [2]). The other variable prefixed URX (ex., URXAAM) provides the analytic result for the analyte. All data are rounded to three significant figures or three decimal places, whichever is less precise.

The lower limit of detection (LLOD, in ng/mL) for urinary VOC metabolites:

VARIABLE NAME	SAS LABEL	LLOD
URXAAM	N-Acetyl-S-(2-carbamoylethyl)-L-cysteine (ng/mL)	2.20
URXAMC	N-Acetyl-S-(N-methylcarbamoyl)-L-cysteine (ng/mL)	6.26
URXATC	2-Aminothiazoline-4-carboxylic acid (ng/mL)	15.0
URXBMA	N-Acetyl-S-(benzyl)-L-cysteine (ng/mL)	0.500
URXBPM	N-Acetyl-S-(n-propyl)-L-cysteine (ng/mL)	1.20
URXCEM	N-Acetyl-S-(2-carboxyethyl)-L-cysteine (ng/mL)	6.96
URXCYHA	N-Acetyl-S-(1-cyano-2-hydroxyethyl)-L-cysteine (ng/mL)	2.60
URXCYM	N-Acetyl-S-(2-cyanoethyl)-L-cysteine (ng/mL)	0.500
URX1DC	N-Acetyl-S-(1,2-dichlorovinyl)-L-cysteine (ng/mL)	12.6
URX2DC	N-Acetyl-S-(2,2-dichlorovinyl)-L-cysteine (ng/mL)	13.9
URXDHB	N-Acetyl-S-(3,4-dihydroxybutyl)-L-cysteine (ng/mL)	5.25
URXDPM	N-Acetyl-S-(dimethylphenyl)-L-cysteine (ng/mL)	0.500
URXGAM	N-Acetyl-S-(2-carbamoyl-2-hydroxyethyl)-L-cysteine (ng/mL)	9.40
URXHEM	N-Acetyl-S-(2-hydroxyethyl)-L-cysteine (ng/mL)	0.791
URXHPM	N-Acetyl-S-(3-hydroxypropyl)-L-cysteine (ng/mL)	13.0
URXHP2	N-Acetyl-S-(2-hydroxypropyl)-L-cysteine (ng/mL)	5.30
URXIPM1	N-Acetyl-S-(2-hydroxy-3-methyl-3-butenyl)-L-cysteine + N-Acetyl-S-(2-hydroxy-2-methyl-3-butenyl)-L-cysteine	1.43
URXIPM3	N-Acetyl- S- (4- hydroxy- 2- methyl- 2- butenyl)- L- cysteine (ng/mL)	1.20
URXMAD	Mandelic acid (ng/mL)	12.0
URX2MH	2-Methylhippuric acid (ng/mL)	5.00
URX34M	3- and 4-Methylhippuric acid (ng/mL)	8.00
URXMB1	N-Acetyl-S-(1-hydroxymethyl-2-propenyl)-L-cysteine (ng/mL)	0.700
URXMB2	N-Acetyl-S-(2-hydroxy-3-butenyl)-L-cysteine (ng/mL)	0.700
URXMB3	N-Acetyl-S-(4-hydroxy-2-butenyl)-L-cysteine (ng/mL)	0.600
URXPHE	N-Acetyl-S-(phenyl-2-hydroxyethyl)-L-cysteine (ng/mL)	0.700
URXPHG	Phenylglyoxylic acid (ng/mL)	12.0
URXPMA	N-Acetyl-S-(phenyl)-L-cysteine (ng/mL)	0.600
URXPMM	N-Acetyl-S-(3-hydroxypropyl-1-methyl)-L-cysteine (ng/mL)	3.00
URXTCV	N-Acetyl-S-(trichlorovinyl)-L-cysteine (ng/mL)	3.00

  

Please refer to the NHANES Analytic Guidelines and the on-line NHANES Tutorial for further details on the use of sample weights and other analytic issues.

References

• Alwis KU, Blount BC, Britt AS, Patel D, Ashley DL. 2012. Simultaneous analysis of 28 urinary VOC metabolites using ultra high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI/MSMS). Anal Chim Acta 750:152-160.
• Caudill SP, Schleicher RL, Pirkle JL. Multi-rule quality control for the age-related eye disease study. Stat Med 2008;27:4094-106.
• Westgard J.O., Barry P.L., Hunt M.R., Groth T. A multi-rule Shewhart chart for quality control in clinical chemistry. Clin Chem (1981) 27:493-501.

Codebook and Frequencies