Table of Contents

Component Description

Glyphosate is the most widely used herbicide in the United States in the agricultural sector and the second most used in the home and garden market sector (U.S. EPA, 2017). Its use has increased more than 200-fold since 1974 (Benbrook, 2016). Glyphosate is widely present in the ecosystem, and it has been detected in a variety of foods (Soares et. al., 2021). Scientific evidence suggests harmful effects of glyphosate in several animal models; however, the evidence on human health effects is limited and controversial (Myers et. al., 2016). In 2015, the International Agency for Research on Cancer determined that glyphosate is a “probable human carcinogen” (IARC, 2015). In contrast, the European Food Safety Authority and the Joint Food and Agriculture Organization (FAO)/World Health Organization (WHO) Meeting on Pesticide Residues (EFSA, 2015; FAO/WHO, 2015) determined that glyphosate is unlikely to be a carcinogen. The U.S. EPA concluded that “available data and weight-of-evidence clearly do not support the descriptors “carcinogenic to humans,” “likely to be carcinogenic to humans,” or “inadequate information to assess carcinogenic potential” (U.S. EPA, 2017a). Similarly, the European Union’s Assessment Group on Glyphosate (EUAGG, 2021) concluded that glyphosate is safe for all proposed uses when used as directed and proposed to declassify it as carcinogenic. Results from two previous cycles of NHANES have recently demonstrated widespread exposure to glyphosate in the U.S. population (Ospina et. al., 2022; CDC, 2023).

Eligible Sample

All examined participants aged 3 to 5 years and a one-third subsample of examined participants aged 6 years and older were eligible.

Description of Laboratory Methodology

This method used 200 microliters of urine and was based on 2D-on-line ion chromatography coupled with tandem mass spectrometry (IC-MS/MS) and isotope dilution quantification (Schütze et. al., 2021).

There were no changes to the lab method, lab equipment, or lab site for this component in the NHANES 2017-2018 cycle. The detection limits did change in the NHANES 2017-2018 cycle.   

Laboratory Quality Assurance and Monitoring

The analytical measurements were conducted following strict quality control/quality assurance CLIA guidelines. Along with the study samples, each analytical run included high- and low-concentration quality control materials (QCMs) and reagent blanks to assure the accuracy and reliability of the data. The concentrations of the high-concentration QCMs and the low-concentration QCMs, averaged to obtain one measurement of high-concentration QCM and low-concentration QCM for each run, were evaluated using standard statistical probability rules (Caudill et. al., 2008).

Data Processing and Editing

Data were received after all analyses were complete. The data were not edited. The data were reviewed. Incomplete data or improbable values were sent to the performing laboratory for confirmation.

Analytic Notes

Refer to the 2017 – 2018 Laboratory Data Overview for general information on NHANES laboratory data.

There are over 800 laboratory tests performed on NHANES participants. However, not all participants provided biospecimens or enough volume for all the tests to be performed. Additionally, availability of specimens for surplus projects is lower than for other laboratory tests performed on NHANES participants. The specimen availability can also vary by age or other population characteristics. Analysts should evaluate the extent of missing data in the dataset related to the outcome of interest as well as any predictor variables used in the analyses to determine whether additional re-weighting for item non-response is necessary.

Please refer to the NHANES Analytic Guidelines and the on-line NHANES Tutorial for further details on the use of sample weights and other analytic issues.

Subsample Weights

The analytes included in this dataset were measured in all examined participants aged 3-5 years, and in a one-third subsample of participants 6 years and older. Special sample weights are required to analyze these data properly. Specific sample weights for this subsample, WTSSBJ2Y, are included in this data file and should be used when analyzing these data. The sample weights created for this file used the examination sample weight, i.e., WTMEC2YR, as the base weight. The base weight was adjusted for additional nonresponse to these lab tests and re-poststratified to the population total using sex, age, and race/Hispanic origin. Participants who were part of the eligible population but who did not provide a urine specimen, or did not have sufficient volume of biospecimens, or who did not give consent for their specimens to be used for future research are included in the file, but they have a sample weight assigned “0” in their records.

Demographic and Other Related Variables

The analysis of NHANES laboratory data must be conducted using the appropriate survey design and demographic variables. The NHANES 2017-2018 Demographics File contains demographic data, health indicators, and other related information collected during household interviews as well as the sample design variables. The recommended procedure for variance estimation requires use of stratum and PSU variables (SDMVSTRA and SDMVPSU, respectively) in the demographic data file.

This laboratory data file can be linked to the other NHANES data files using the unique survey participant identifier (i.e., SEQN).

The variable URXUCR (urine creatinine) will not be reported in this file. URXUCR can be found in the data file titled “Albumin & Creatinine – Urine”.

Detection Limits

The detection limit was constant for all of the analytes in the data set. Two variables are provided for each of these analytes. The variable name ending in “L” (ex., SSGLYPL) indicates whether the result was below the limit of detection: the value “0” means that the result was at or above the limit of detection, “1” indicates that the result was below the limit of detection. The other variable (ex., SSGLYP) provides the analytic result for that analyte. For analytes with analytic results below the lower limit of detection (ex., SSGLYPL=1), an imputed fill value was placed in the analyte results field. This value is the lower limit of detection divided by square root of 2 (LLOD/sqrt[2]). The lower limit of detection (LLOD, in ng/mL) for GLYP:

Variable Name    Analyte Description LLOD
 SSGLYP  Glyphosate (ng/mL)  0.1

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 3 YEARS - 150 YEARS

WTSSBJ2Y - Surplus specimen B 17-18 2 yr weights

Variable Name:
WTSSBJ2Y
SAS Label:
Surplus specimen B 17-18 2 yr weights
English Text:
Surplus specimen 2 year weights B for 2017-2018
Target:
Both males and females 3 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
5978.513209 to 1489188.6434 Range of Values 2331 2331
0 No lab specimen 655 2986
. Missing 0 2986

SSGLYP - Glyphosate (ng/mL)

Variable Name:
SSGLYP
SAS Label:
Glyphosate (ng/mL)
English Text:
Glyphosate (ng/mL)
Target:
Both males and females 3 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.071 to 7.69 Range of Values 2329 2329
. Missing 657 2986

SSGLYPL - Glyphosate comment code

Variable Name:
SSGLYPL
SAS Label:
Glyphosate comment code
English Text:
Glyphosate comment code
Target:
Both males and females 3 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 1922 1922
1 Below lower detection limit 407 2329
. Missing 657 2986