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Panel:   AR-1306 & AR-1307 S.aureus mecC+ (Custom)
AR Bank # 1306 Staphylococcus aureus
Study ID: BOR-33

Biosample Accession #: Biosample Accession data is not yet available.

   
Location:
Denmark
Source:
nose/throath
MLST: ST130(Pasteur)


Panel:  Staphylococcus with Borderline Oxacillin Susceptibility (BORSA)  |  AR-1306 & AR-1307 S.aureus mecC+ (Custom)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Cefoxitin 16R
Ceftaroline <=0.5S
Clindamycin <=0.25S
Daptomycin <=0.5---
Doxycycline <=1S
Erythromycin 0.5S
Levofloxacin <=0.5---
Linezolid 4---
Mupirocin <=4S
Oxacillin 2S
Penicillin 1R
Rifampin <=0.5S
Tetracycline <=1S
Trimethoprim/sulfamethoxazole 1<=0.5S
S – I –R Interpretation (INT) derived from CLSI 2024 M100 S34

1 Reflects MIC of first component
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aph-STPH
Beta-lactam blaZ-mecC
Efflux pumps/Other DHA-1
Tetracyclines tet(38)
 
Disclaimer:
The resistance mechanisms listed were identified by analysis of whole genome sequence using the ResFinder database (last updated June 2, 2016 and accessed on October 25, 2016). This analysis does not include mutations that may result in antibiotic resistance or resistance determinants added to newer versions of the ResFinder database or other antimicrobial resistance gene databases. Biosample accession numbers have been provided so that users can analyze the data on their own if so desired.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.

Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.

Disclaimer:
This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
01/03/25MMR was changed: from 'Antimicrobial Resistance (AR) gene prediction was performed using a combined and deduplicated AR database (ResGANNCBI) from ResFinder, ARG-ANNOT, and NCBI AMRFinder(primary Database) accessed on 2021-05-07. AR drug classes are assigned according to these databases. This analysis does not include mutations that may result in antimicrobial resistance, or resistance determinants added to newer versions of databases used, or other antimicrobial resistance gene databases. Additional AR genes other than those listed, may be present. For resistance determinant detection, 99-100% sequence identity and 100% sequence coverage from GAMMA (primary AR gene calling tool) and SRST2 was used. GAMMA uses amino acid sequence to assign gene alleles from assemblies; SRST2 uses nucleotide sequence to assign gene alleles from sequencing reads and is used to resolve AR gene calls located on the edges of assembly scaffolds detected by GAMMA. Biosample accession numbers have been provided so that users can analyze the raw sequencing data on their own, if so desired. *MLST Type (and scheme), as determined by Torsten Seemann's MLST program. For Enterobacterales the Pasteur MLST schemes are used except for E. coli for which both the Pasteur and Achtman schemes are reported. Similarly, for Acinetobacter baumannii complex, both Pasteur and Oxford MLST schemes are used and reported. SUB=novel MLST identified and "submitted". Dash (-) indicates no pubMLST scheme is available for that species. Truncated porins are defined as porin genes detected with ≥80% sequence identity and ≥80% sequence coverage and predicted to have an internal stop codon by c-SSTAR (https://github.com/chrisgulvik/c-SSTAR). ** Prescence of Efflux Pump may not be associated with resistance.' to 'The resistance mechanisms listed were identified by analysis of whole genome sequence using the ResFinder database (last updated June 2, 2016 and accessed on October 25, 2016). This analysis does not include mutations that may result in antibiotic resistance or resistance determinants added to newer versions of the ResFinder database or other antimicrobial resistance gene databases. Biosample accession numbers have been provided so that users can analyze the data on their own if so desired.'
01/03/25New Isolate added to panel
01/03/25INT was updated with a new value for Trimethoprim/sulfamethoxazole: from '---' to 'S'
01/03/25MIC was updated with a new value for Trimethoprim/sulfamethoxazole: from '---' to '<=0.5'
01/03/25MIC was updated with a new value for Erythromycin: from '---' to '0.5'
01/03/25Drug was added: 'Trimethoprim/sulfamethoxazole'
01/03/25INT was updated with a new value for Erythromycin: from '---' to 'S'
01/03/25MIC was updated with a new value for Trimethoprim/sulfamethoxazole: from '---' to '<=0.5'
01/03/25MIC was updated with a new value for Erythromycin: from '---' to '0.5'
01/03/25Drug was added: 'Erythromycin'
01/03/25INT was updated with a new value for Tetracycline: from '---' to 'S'
01/03/25INT was updated with a new value for Linezolid: from '---' to '---'
01/03/25INT was updated with a new value for Doxycycline: from '---' to 'S'
01/03/25MIC was updated with a new value for Tetracycline: from '---' to '<=1'
01/03/25MIC was updated with a new value for Linezolid: from '---' to '4'
01/03/25MIC was updated with a new value for Doxycycline: from '---' to '<=1'
01/03/25Drug was added: 'Tetracycline'
01/03/25Drug was added: 'Linezolid'
01/03/25Drug was added: 'Doxycycline'
01/03/25INT was updated with a new value for Rifampin: from '---' to 'S'
01/03/25MIC was updated with a new value for Rifampin: from '---' to '<=0.5'
01/03/25MIC was updated with a new value for Penicillin: from '---' to '1'
01/03/25MIC was updated with a new value for Levofloxacin: from '---' to '<=0.5'
01/03/25MIC was updated with a new value for Daptomycin: from '---' to '<=0.5'
01/03/25MIC was updated with a new value for Ceftaroline: from '---' to '<=0.5'
01/03/25Drug was added: 'Rifampin'
01/03/25INT was updated with a new value for Penicillin: from '---' to 'R'
01/03/25INT was updated with a new value for Levofloxacin: from '---' to '---'
01/03/25INT was updated with a new value for Daptomycin: from '---' to '---'
01/03/25INT was updated with a new value for Ceftaroline: from '---' to 'S'
01/03/25MIC was updated with a new value for Rifampin: from '---' to '<=0.5'
01/03/25MIC was updated with a new value for Penicillin: from '---' to '1'
01/03/25MIC was updated with a new value for Levofloxacin: from '---' to '<=0.5'
01/03/25MIC was updated with a new value for Daptomycin: from '---' to '<=0.5'
01/03/25MIC was updated with a new value for Ceftaroline: from '---' to '<=0.5'
01/03/25Drug was added: 'Penicillin'
01/03/25Drug was added: 'Levofloxacin'
01/03/25Drug was added: 'Daptomycin'
01/03/25Drug was added: 'Ceftaroline'
01/03/25INT was updated with a new value for Mupirocin: from '---' to 'S'
01/03/25INT was updated with a new value for Clindamycin: from '---' to 'S'
01/03/25MIC was updated with a new value for Mupirocin: from '---' to '<=4'
01/03/25MIC was updated with a new value for Clindamycin: from '---' to '<=0.25'
01/03/25Drug was added: 'Mupirocin'
01/03/25Drug was added: 'Clindamycin'
01/03/25Gene was added to Beta-lactam: 'blaZ-mecC '
01/03/25Category was added: Beta-lactam
01/03/25INT was updated with a new value for Cefoxitin: from '---' to 'R'
01/03/25MIC was updated with a new value for Cefoxitin: from '---' to '16'
01/03/25Drug was added: 'Cefoxitin'
01/03/25INT was updated with a new value for Oxacillin: from '---' to 'S'
01/03/25MIC was updated with a new value for Oxacillin: from '---' to '2'
01/03/25Drug was added: 'Oxacillin'
01/03/25Source was changed: from '---' to 'nose/throath'
01/03/25Location was changed: from '---' to 'Denmark'
01/03/25Gene was added to Efflux pumps/Other: 'DHA-1'
01/03/25Gene was added to Tetracyclines: 'tet(38)'
01/03/25Gene was added to Aminoglycoside: 'aph-STPH'
01/03/25Category was added: Efflux pumps/Other
01/03/25Category was added: Tetracyclines
01/03/25Category was added: Aminoglycoside
01/03/25MLST was updated: from '---' to 'ST130(Pasteur)'
01/03/25Drug was removed: Mupirocin
01/03/25Drug was removed: Colistin
01/03/25Drug was removed: Amphotericin B
01/03/25Drug was removed: Amikacin
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