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Panel:   IMP-Type Metallo-ß-Lactamase (IMP)
AR Bank # 1334 Pseudomonas aeruginosa
Study ID: IMP-12

Biosample Accession #: Biosample Accession data is not yet available.

   

Collection Year:
2022
Location:
USA
Source:
P-trap water
MLST: ST235(Pasteur)

MICs obtained by broth microdilution. Modal MIC is reported.

MIC results for each antimicrobial agent for an isolate may commonly be ± 1 log2 (doubling dilution) different than what is posted on the FDA & CDC AR Bank website because this is the normal technical variability of antimicrobial susceptibility testing (see J. H. Jorgensen. 1993. J Clin Microbiol. Vol 31[11]: 2841-2844).

Panel:  IMP-Type Metallo-ß-Lactamase (IMP)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Amikacin ---
Ceftazidime/avibactam 1---
S – I –R Interpretation (INT) derived from CLSI 2025 M100 S35
S – I –R Interpretation (INT) derived from CLSI 2026 M100 S36
SDD (Susceptible Dose Dependent)

1 Reflects MIC of first component
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aac(6')-IIa, aacA4, aadA5, aph(3")-Ib, aph(3')-IIb, aph(6)-Id
Beta-lactam IMP-84, OXA-2, OXA-4, OXA-488, PDC-35
Efflux pumps/Other mexA, mexE, qacEdelta1
Fosfomycin fosA
Macrolide-Lincosamide-Streptogramin ere(A), mph(E), msr(E)
Phenicols/Bicyclomycins bcr1
Sulfonamides sul1
Trimethoprim dfrA5
 
Disclaimer:

Antimicrobial Resistance (AR) gene prediction was performed using a combined and deduplicated AR database (ResGANNCBI) from ResFinder, ARG-ANNOT, and NCBI AMRFinder(primary Database) accessed on 2022-09-15. AR drug classes are assigned according to these databases. This analysis does not include mutations that may result in antimicrobial resistance, or resistance determinants added to newer versions of databases used, or other antimicrobial resistance gene databases. Additional AR genes other than those listed, may be present. For resistance determinant detection, 99-100% sequence identity and 100% sequence coverage from GAMMA (primary AR gene calling tool) and SRST2 was used. GAMMA uses amino acid sequence to assign gene alleles from assemblies; SRST2 uses nucleotide sequence to assign gene alleles from sequencing reads and is used to resolve AR gene calls located on the edges of assembly scaffolds detected by GAMMA. Biosample accession numbers have been provided so that users can analyze the raw sequencing data on their own, if so desired. *MLST Type (and scheme), as determined by Torsten Seemann's MLST program. For Enterobacterales the Pasteur MLST schemes are used except for E. coli for which both the Pasteur and Achtman schemes are reported. Similarly, for Acinetobacter baumannii complex, both Pasteur and Oxford MLST schemes are used and reported. SUB=novel MLST identified and "submitted". Dash (-) indicates no pubMLST scheme is available for that species. Truncated porins are defined as porin genes detected with =80% sequence identity and =80% sequence coverage and predicted to have an internal stop codon by c-SSTAR (https://github.com/chrisgulvik/c-SSTAR). ** Prescence of Efflux Pump may not be associated with resistance. For additional gene information please visit the Reference Gene Catalog - Pathogen Detection - NCBI, a non-redundant database of bacterial genes related to antimicrobial resistance, biocide and stress resistance, general efflux, virulence, or antigenicity.

Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP); Brain Heart Infusion Agar with 5% Rabbit Blood (BHIA)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Ambient

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.

Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.

Isolate History
Date Action Performed
04/10/26Gene was added to Efflux pumps/Other: 'qacEdelta1'
04/10/26Gene was added to Efflux pumps/Other: 'mexE'
04/10/26Gene was added to Efflux pumps/Other: 'mexA'
04/10/26Gene was added to Trimethoprim: 'dfrA5'
04/10/26Gene was added to Sulfonamides: 'sul1'
04/10/26Gene was added to Phenicols/Bicyclomycins: 'bcr1'
04/10/26Gene was added to Macrolide-Lincosamide-Streptogramin: 'msr(E)'
04/10/26Gene was added to Macrolide-Lincosamide-Streptogramin: 'mph(E)'
04/10/26Gene was added to Macrolide-Lincosamide-Streptogramin: 'ere(A)'
04/10/26Gene was added to Fosfomycin: 'fosA'
04/10/26Gene was added to Aminoglycoside: 'aph(6)-Id'
04/10/26Gene was added to Aminoglycoside: 'aph(3')-IIb'
04/10/26Gene was added to Aminoglycoside: 'aph(3")-Ib'
04/10/26Gene was added to Aminoglycoside: 'aadA5'
04/10/26Gene was added to Aminoglycoside: 'aacA4'
04/10/26Gene was added to Aminoglycoside: 'aac(6')-IIa'
04/10/26Gene was added to Beta-lactam: 'IMP-84'
04/10/26Category was added: Efflux pumps/Other
04/10/26Category was added: Trimethoprim
04/10/26Category was added: Sulfonamides
04/10/26Category was added: Phenicols/Bicyclomycins
04/10/26Category was added: Macrolide-Lincosamide-Streptogramin
04/10/26Category was added: Fosfomycin
04/10/26Category was added: Aminoglycoside
04/10/26MLST was updated: from '---' to 'ST235(Pasteur)'
04/10/26Source was changed: from '---' to 'P-trap water'
04/10/26Location was changed: from '---' to 'USA'
04/10/26Collection Year was changed: from '---' to '2022'
04/10/26Gene was added to Beta-lactam: 'PDC-35'
04/10/26Gene was added to Beta-lactam: 'OXA-488'
04/10/26Gene was added to Beta-lactam: 'OXA-4'
04/10/26Gene was added to Beta-lactam: 'OXA-2'
04/10/26Category was added: Beta-lactam
07/24/25Isolate Created
07/24/25Drug was added: 'Ceftazidime/avibactam'
07/24/25Drug was added: 'Amikacin'
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