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Panel:   GN7F ARLN (Custom)
AR Bank # 0085 Escherichia coli
Study ID: CarbaNP-54

Biosample Accession #: SAMN04014926

mCIM Result: Negative    Carba NP Result: Negative
MLST: Unknown(Pasteur), ST4(Achtman)


Panel:  Gram Negative Carbapenemase Detection (CarbaNP)  |  ARLN Verification (Custom)  |  GN7F ARLN (Custom)  |  GAIHN (Custom)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Amikacin 8I
Ampicillin >32R
Ampicillin/sulbactam 1>32R
Aztreonam 64R
Cefazolin >8R
Cefepime 2S
Cefotaxime 32R
Cefotaxime/clavulanic acid 1>32---
Cefoxitin >16R
Ceftazidime 128R
Ceftazidime/avibactam 1<=0.5S
Ceftazidime/clavulanic acid 1>64---
Ceftriaxone >32R
Ciprofloxacin >8R
Colistin 40.5I
Doripenem <=0.12S
Eravacycline 0.12S
Ertapenem 2R
Gentamicin 0.5S
Imipenem <=0.5S
Imipenem+chelators 30.5---
Levofloxacin >8R
Meropenem 1S
Minocycline 16R
Piperacillin/tazobactam 164R
Plazomicin 1S
Tetracycline >32R
Tigecycline 2<=0.5S
Tobramycin 1S
Trimethoprim/sulfamethoxazole 1>8R
S – I –R Interpretation (INT) derived from CLSI 2024 M100 S34
1 Reflects MIC of first component
2 Based on FDA break points
3 Screen for metallo-beta-lactamase production [Rasheed et al. Emerging Infectious Diseases. 2013. 19(6):870-878]
4 Clinical and PK/PD data demonstrate colistin has limited clinical efficacy, even if an intermediate result is obtained. Alternative agents are strongly preferred. Colistin should be used in combination with one or more active antimicrobial agents. Consultation with an infectious disease specialist is recommended.
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aadA1, aph(3")-Ib, aph(6)-Id
Beta-lactam CMY-2
Efflux pumps/Other EMRD, MDTM
Phenicols/Bicyclomycins floR
Streptothricin sat2
Sulfonamides sul2
Tetracyclines tet(A), tet(B), tet(R)
Trimethoprim dfrA1
Truncated Porin OmpF
 
Disclaimer:
The resistance mechanisms listed were identified by analysis of whole genome sequence using the ResFinder database (last updated June 2, 2016 and accessed on October 25, 2016). This analysis does not include mutations that may result in antibiotic resistance or resistance determinants added to newer versions of the ResFinder database or other antimicrobial resistance gene databases. Biosample accession numbers have been provided so that users can analyze the data on their own if so desired.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.

Disclaimer:
This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
04/20/23MIC was updated with a new value for Eravacycline: from '0.25' to '0.12'
04/10/23INT was updated with a new value for Amikacin: from 'S' to 'I'
03/28/23Gene was added to 'MDTM'
03/28/23Gene was added to 'EMRD'
03/28/23Gene was added to 'sat2'
03/28/23Gene was added to 'floR'
03/28/23Gene was added to 'tet(R)'
03/28/23Gene was added to 'tet(A)'
03/28/23Gene was added to 'aph(6)-Id'
03/28/23Gene was added to 'aph(3")-Ib'
03/28/23Category was added: 'Efflux pumps/Other'
03/28/23Category was added: 'Streptothricin'
03/28/23Category was added: 'Phenicols/Bicyclomycins'
03/28/23Gene was removed from 'from '---' to ' strB''
03/28/23Gene was removed from 'from '---' to ' strA''
12/19/22MLST was updated: from '---' to 'ST4(Achtman)'
12/19/22MLST was updated: from '---' to 'Unknown(Pasteur)'
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