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Panel:   Neisseria gonorrhoeae Reference (NG)
AR Bank # 0914 Neisseria gonorrhoeae
Study ID: NG-Z

Biosample Accession #: Biosample Accession data is not yet available.

β-Lactamase: Negative*

*April 1, 2025

Neisseria gonorrhoeae Reference Isolates is the new name of a pre-existing panel, with similar name, provided through the AR Isolate Bank. The identification (ID) convention for the isolates in this panel has also changed. The changes are applicable only to the naming of the panel and the isolate IDs. The changes do not affect the physical characteristics of any reference gonococcal strains in this panel. The changes are as follow:

        i)           New name for this panel is Neisseria gonorrhoeae Reference Isolates.

      ii)           New isolate IDs are NG-F, NG-G, NG-K, NG-L, NG-M, NG-N, NG-O, NG-P, NG-U, NG-V, NG-W, NG-X, NG-Y, and NG-Z.

The ‘unique letter’ designation in the isolate IDs (F, G, K, L, M, N, O, P, U, V, W, X, Y, and Z) is transferred over from the previous panel’s isolate IDs for each isolate (i.e., the ‘new’ Z is the same strain as the ‘old’ Z). The AR Isolate Bank Number for the gonococcal strains in this panel are retained and could be used to link back to the previous naming convention for each isolate. Lastly, the ‘unique letter’ in the isolate IDs corresponds to the same strain with the same letter assignment published by Unemo et.al. (2016)#. For questions, or to request additional information about this announcement, please contact the CDC & FDA AR Isolate Bank via email at ARbank@cdc.gov.

#https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5079299/


MIC results provided were determined by agar dilution.

MIC results for each antimicrobial agent for an isolate may commonly be ± 1 log2 (doubling dilution) different than what is posted on the FDA & CDC AR Bank website because this is the normal technical variability of antimicrobial susceptibility testing (see J. H. Jorgensen. 1993. J Clin Microbiol. Vol 31[11]: 2841-2844).

Panel:  Neisseria gonorrhoeae Reference (NG)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Azithromycin 1S
Cefixime >1---
Ceftriaxone 0.5---
Ciprofloxacin 32R
Gentamicin 8---
Penicillin 4R
Tetracycline 2R
* Phenotypic test
S – I –R Interpretation (INT) derived from CLSI 2024 M100 S34
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
 
Disclaimer:

Biosample accession numbers have been provided so that users can analyze the data on their own if so desired.
Propagation
MEDIUM
Medium: GC agar base and 1% defined growth supplement

GROWTH CONDITIONS
Temperature: 36°C
Atmosphere: 5% CO₂

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to GC agar

Use streak plate method to isolate single colonies

Incubate inverted plate at 36°C ± 1°C in 5% CO₂ for 20-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
10/02/23INT was updated with a new value for Azithromycin: from '---' to 'S'
01/04/19New Isolate added to panel
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