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Panel:   Isolates with New or Novel Antibiotic Resistance (NEW)
AR Bank # 1270 Pseudomonas aeruginosa
Study ID: NEW-36

Biosample Accession #: SAMN33435243


Collection Year:
MLST: 1203

MICs obtained by broth microdilution. Modal MIC is reported.

MIC results for each antimicrobial agent for an isolate may commonly be ± 1 log2 (doubling dilution) different than what is posted on the FDA & CDC AR Bank website because this is the normal technical variability of antimicrobial susceptibility testing (see J. H. Jorgensen. 1993. J Clin Microbiol. Vol 31[11]: 2841-2844).

Note: AR- 0349 Escherichia coli  has been removed from the collection and is now available at ATCC under catalog # BAA-3170

Panel:  Isolates with New or Novel Antibiotic Resistance (NEW)

MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Amikacin >64R
Aztreonam >32R
Cefepime >32R
Cefiderocol 0.5S
Ceftazidime >128R
Ceftazidime/avibactam 1>16R
Ceftolozane/tazobactam 1>16R
Ciprofloxacin >8R
Colistin 21I
Imipenem 16R
Imipenem/relebactam 18R
Imipenem+chelators 32---
Levofloxacin >8R
Meropenem >8R
Piperacillin/tazobactam 164R
Tobramycin >16R
S – I –R Interpretation (INT) derived from CLSI 2023 M100 S33

1 Reflects MIC of first component
2 Clinical and PK/PD data demonstrate colistin has limited clinical efficacy, even if an intermediate result is obtained. Alternative agents are strongly preferred. Colistin should be used in combination with one or more active antimicrobial agents. Consultation with an infectious disease specialist is recommended.
3 Screen for metallo-beta-lactamase production [Rasheed et al. Emerging Infectious Diseases. 2013. 19(6):870-878]
Molecular Mechanisms of Resistance
Aminoglycoside aac(6')-Ib-G, aadA1, aph(3")-Ib, aph(3')-IIb, rmtF2
Beta-lactam GES-9, OXA-10, OXA-395, PDC-19A, VIM-80
Efflux pumps/Other mexA, mexE, qacEdelta1
Fosfomycin fosA
Macrolide-Lincosamide-Streptogramin ere(A)
Phenicols/Bicyclomycins bcr1, catB, floR, floR2
Sulfonamides sul1
Tetracyclines tet(G)
Trimethoprim dfrA5
AR gene prediction was performed using ARG-ANNOT, ResFinder and NCBI (ResGANNCBI) databases accessed on 2021-05-07. AR drug classes are assigned according to these databases. This analysis does not include mutations that may result in antibiotic resistance or resistance determinants added to newer versions of used database or other antimicrobial resistance gene databases. For resistance determinant detection, 99-100% sequence identity and 100% sequence coverage from GAMMA and SRST2 was used. GAMMA uses amino acid sequence to assign gene alleles from assemblies; SRST2 uses nucleotide sequence to assign gene alleles from sequencing reads. Biosample accession numbers have been provided so that users can analyze the data on their own if so desired. *Presence of Efflux Pump may not be associated with resistance. *MLST Type (and scheme), as determined by Torsten Seemann's MLST program. For Enterobacteriaceae, the Pasteur MLST schemes are used except for E. coli for which both the Pasteur and Achtman schemes are reported. SUB=novel MLST identified and "submitted".
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

Temperature: 35°C
Atmosphere: Aerobic


Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.

Storage Temperature & Biosafety

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health. Please Note: This AR Bank panel includes isolates that are pan-resistant, meaning they are susceptible to none of the antimicrobial agents tested which may include those commonly used to treat infections with these organisms. This represents an additional risk to staff working with these organisms and should be considered as part of the receiving facility risk assessment required by the Biosafety Compliance Agreement. The increased difficulty in treatment of any infection with those isolates should also be a consideration in deciding which protocols to use and precautions to take for working with these isolates.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
06/14/23Drug was removed: Gentamicin
04/12/23Interpretation was updated with a new value for Piperacillin/tazobactam: from I to R
02/27/23Sequence Accession # changed: from --- to SAMN33435243
02/24/23Gene was added: qacEdelta1
02/24/23Gene was added: mexE
02/24/23Gene was added: mexA
02/24/23Gene was added: dfrA5
02/24/23Gene was added: tet(G)
02/24/23Gene was added: sul1
02/24/23Gene was added: floR2
02/24/23Gene was added: floR
02/24/23Gene was added: catB
02/24/23Gene was added: bcr1
02/24/23Gene was added: ere(A)
02/24/23Gene was added: fosA
02/24/23Gene was added: rmtF2
02/24/23Gene was added: aph(3')-IIb
02/24/23Gene was added: aph(3")-Ib
02/24/23Gene was added: aadA1
02/24/23Gene was added: aac(6')-Ib-G
02/24/23Gene was added: PDC-19A
02/24/23Gene was added: OXA-395
02/24/23Gene was added: OXA-10
02/24/23Category was added: Efflux pumps/Other
02/24/23Category was added: Trimethoprim
02/24/23Category was added: Tetracyclines
02/24/23Category was added: Sulfonamides
02/24/23Category was added: Phenicols/Bicyclomycins
02/24/23Category was added: Macrolide-Lincosamide-Streptogramin
02/24/23Category was added: Fosfomycin
02/24/23Category was added: Aminoglycoside
02/22/23Collection Year was changed: from --- to 2022
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