Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)
Open vial aseptically to avoid contamination
Using a sterile loop, remove a small amount of frozen isolate from the top of the vial
Aseptically transfer the loop to BAP
Use streak plate method to isolate single colonies
Incubate inverted plate at
35°C ± 2°C
for 18-24 hrs.