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Panel:   Enteric Pathogen Diversity (GI)
AR Bank # 0409 Salmonella Typhimurium
Study ID: GI-09

Biosample Accession #: SAMN12648959

   

MICs obtained by broth microdilution. Modal MIC is reported.

MIC results for each antimicrobial agent for an isolate may commonly be ± 1 log2 (doubling dilution) different than what is posted on the FDA & CDC AR Bank website because this is the normal technical variability of antimicrobial susceptibility testing (see J. H. Jorgensen. 1993. J Clin Microbiol. Vol 31[11]: 2841-2844).

Panel:  Enteric Pathogen Diversity (GI)  |  AR-0387, AR-0401-0426 (Custom)  |  7216 GI Custom (Custom)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Amoxicillin/clavulanic acid 1<=1S
Ampicillin <=1S
Azithromycin 4---
Cefoxitin 8S
Ceftiofur 1---
Ceftriaxone <=0.25S
Chloramphenicol 4S
Ciprofloxacin <=0.015S
Gentamicin <=0.25S
Nalidixic acid 4---
Streptomycin 4---
Sulfisoxazole 32S
Tetracycline <=4S
Trimethoprim/sulfamethoxazole 1<=0.12S
S – I –R Interpretation (INT) derived from CLSI 2021 M100 S31

1 Reflects MIC of first component
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
 
Disclaimer:
For resistance determinant detection, 90% sequence identity and 60% sequence coverage was used. Biosample accession numbers have been provided so that users can analyze the data on their own if so desired. (M) = denotes mutational - gene and position (amino acid) are listed 23S - nucleotide 2075
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP).

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE
Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)
Open vial aseptically to avoid contamination
Using a sterile loop, remove a small amount of frozen isolate from the top of the vial
Aseptically transfer the loop to BAP
Use streak plate method to isolate single colonies
Incubate inverted plate at 35°C ± 2°C for 18-24hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.

Disclaimer:
This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
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