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Panel:   Enteric Pathogen Diversity (GI)
AR Bank # 0415 Campylobacter jejuni
Study ID: GI-15

Biosample Accession #: SAMN12648965


MICs obtained by broth microdilution. Modal MIC is reported.

MIC results for each antimicrobial agent for an isolate may commonly be ± 1 log2 (doubling dilution) different than what is posted on the FDA & CDC AR Bank website because this is the normal technical variability of antimicrobial susceptibility testing (see J. H. Jorgensen. 1993. J Clin Microbiol. Vol 31[11]: 2841-2844).

Panel:  Enteric Pathogen Diversity (GI)  |  AR-0387, AR-0401-0426 (Custom)

MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Azithromycin 0.12WT
Ciprofloxacin 0.12S
Erythromycin 0.5S
Florfenicol 1WT
Gentamicin >32NWT
Nalidixic acid <=4WT
Tetracycline >64R
S – I –R Interpretation (INT) derived from M45 2016 S3
WT (wild type; =ECVs) – NWT (non-wild type; =ECVs) based on epidemiological cutoff values (ECVs) adopted from the European Committee on Antimicrobial Susceptibility Testing (EUCAST, 2021)
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
Aminoglycoside aph(3')-III
Tetracyclines tet(O)
For resistance determinant detection, 90% sequence identity and 60% sequence coverage was used. Biosample accession numbers have been provided so that users can analyze the data on their own if so desired. (M) = denotes mutational - gene and position (amino acid) are listed 23S - nucleotide 2075
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP); Brain Heart Infusion Agar with 5% Rabbit Blood (BHIA)

Temperature: 37°C
Atmosphere: Micro-aerobic

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)
Open vial aseptically to avoid contamination
Using a sterile loop, remove a small amount of frozen isolate from the top of the vial
Aseptically transfer the loop to BHIA
Use streak plate method to isolate single colonies
Incubate inverted plate at 37°C for 24-48 hrs., then subculture to BAP and allow 48 hours of incubation prior to performing Antimicrobial Susceptibility Testing (AST).
Storage Temperature & Biosafety

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
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