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Panel:   Enteric Pathogen Diversity (GI)
AR Bank # 0427 Escherichia coli O157
Study ID: GI-27

Biosample Accession #: SAMN12648977

   
Source: stool

MICs obtained by broth microdilution. Modal MIC is reported.

MIC results for each antimicrobial agent for an isolate may commonly be ± 1 log2 (doubling dilution) different than what is posted on the FDA & CDC AR Bank website because this is the normal technical variability of antimicrobial susceptibility testing (see J. H. Jorgensen. 1993. J Clin Microbiol. Vol 31[11]: 2841-2844).

Panel:  Enteric Pathogen Diversity (GI)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Amoxicillin/clavulanic acid 18S
Ampicillin >32R
Azithromycin 2---
Cefoxitin 8S
Ceftiofur 20.5S
Ceftriaxone <=0.25S
Chloramphenicol >32R
Ciprofloxacin 0.25S
Gentamicin 1S
Nalidixic acid >32R
Streptomycin >64---
Sulfisoxazole >256R
Tetracycline >32R
Trimethoprim/sulfamethoxazole 1>4R
S – I –R Interpretation (INT) derived from CLSI 2022 M100 S32
1 Reflects MIC of first component
2 Based on CLSI 2015 VET01S ED3
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
 
Disclaimer:

For resistance determinant detection, 90% sequence identity and 60% sequence coverage was used. Biosample accession numbers have been provided so that users can analyze the data on their own if so desired.

(M) = denotes mutational - gene and position (amino acid) are listed

23S - nucleotide 2075

For additional gene information please visit the Reference Gene Catalog - Pathogen Detection - NCBI (https://www.ncbi.nlm.nih.gov/pathogens/refgene), a non-redundant database of bacterial genes related to antimicrobial resistance, biocide and stress resistance, general efflux, virulence, or antigenicity.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP).

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE
Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)
Open vial aseptically to avoid contamination
Using a sterile loop, remove a small amount of frozen isolate from the top of the vial
Aseptically transfer the loop to BAP
Use streak plate method to isolate single colonies
Incubate inverted plate at 35°C ± 2°C for 18-24hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
10/13/22INT was updated with a new value for Ciprofloxacin: from 'I' to 'S'
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