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Panel:   AR Bank Custom set (Custom)
AR Bank # 0781 Enterococcus faecalis
Study ID: VRE-01

Biosample Accession #: SAMN15040080
MLST: ST4(Pasteur)

Panel:  Vancomycin-Resistant Enterococci (VRE)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Ampicillin 1S
Chloramphenicol 8S
Daptomycin 4I
Doxycycline 8I
High-Level Gentamicin 1<=500SYN-S
High-Level Streptomycin 1<=1000SYN-S
Levofloxacin 2S
Linezolid 2S
Penicillin 2S
Quinupristin/dalfopristin 24R
Rifampin 1S
Tedizolid 0.25S
Teicoplanin >64R
Vancomycin >64R
S – I –R Interpretation (INT) derived from CLSI 2024 M100 S34
SDD (Susceptible Dose Dependent)
1 SYN-S: is synergistic with cell-wall active agent (e.g., ampicillin, penicillin and vancomycin) if the cell-wall active agent is also susceptible
2 Reflects MIC of the combination
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Glycopeptides VanA
Tetracyclines tet(L), tet(M)
 
Disclaimer:
No disclaimer.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

Isolate History
Date Action Performed
11/20/23Sequence Accession # changed: from '---' to 'SAMN15040080'
06/06/23MLST was updated: from '---' to 'ST4(Pasteur)'
06/05/23Isolate Status changed: from 'Pending' to 'Active'
06/05/23Isolate was removed from panel
06/05/23Isolate Status changed: from 'Active' to 'Pending'
06/05/23Gene was added to 'VanA'
06/05/23Category was added: 'Glycopeptides'
06/05/23Isolate Status changed: from 'Pending' to 'Active'
06/05/23Isolate was removed from panel
06/05/23Isolate Status changed: from 'Active' to 'Pending'
06/05/23Gene was removed from 'from '---' to ' VanB''
06/05/23Category was removed: from '---' to 'Colistin'
06/05/23Gene was added to 'tet(M)'
06/05/23Gene was added to 'tet(L)'
06/05/23Gene was added to 'VanA'
06/05/23Gene was added to 'VanB'
06/05/23Category was added: 'Tetracyclines'
06/05/23Category was added: 'Colistin'
06/05/23Gene was removed from 'from '---' to ' VanA''
02/14/23Isolate Status changed: from 'Pending' to 'Active'
02/14/23Drug was removed: 'Omadacycline'
02/14/23Isolate was removed from panel
02/14/23Isolate Status changed: from 'Active' to 'Pending'
02/14/23INT was updated with a new value for Daptomycin: from 'SDD' to 'I'
02/14/23INT was updated with a new value for Daptomycin: from 'I' to 'SDD'
02/14/23INT was updated with a new value for Tedizolid: from '---' to 'S'
02/14/23MIC was updated with a new value for Tedizolid: from '---' to '0.25'
02/14/23Drug was added: Tedizolid
02/14/23INT was updated with a new value for Omadacycline: from '---' to 'S'
02/14/23MIC was updated with a new value for Omadacycline: from '---' to '0.25'
02/14/23Drug was added: Omadacycline
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