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Panel:   Tedizolid/Linezolid (AR-0781 - 0795) and VRE (AR-0474 - 0488) (Custom)
AR Bank # 0783 Enterococcus faecium
Study ID: VRE-03

Biosample Accession #: SAMN15040082

   
MLST: ST17(Pasteur)


Panel:  Vancomycin-Resistant Enterococci (VRE)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Ampicillin 64R
Chloramphenicol 16I
Daptomycin 4SDD
Doxycycline 16R
High-Level Gentamicin 1>500SYN-R
High-Level Streptomycin 1>1000SYN-R
Levofloxacin 4I
Linezolid 2S
Penicillin >32R
Rifampin >4R
Teicoplanin 2S
Vancomycin 64R
SDD (Susceptible Dose Dependent)
S – I –R Interpretation (INT) derived from CLSI 2025 M100 S35
1 SYN-R: is not synergistic with cell-wall active agent(e.g., ampicillin, penicillin and vancomycin)
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aac(6')-Ie, aacA-ENT1, ant(6)-Ia, aph(3')-IIIa
Efflux pumps/Other eat(A)
Glycopeptides VanB
Streptothricin sat4
Tetracyclines tet(L)
Trimethoprim dfrG
 
Disclaimer:
No disclaimer.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

Isolate History
Date Action Performed
11/20/23Sequence Accession # changed: from '---' to 'SAMN15040082'
06/23/23Gene was added to 'VanB'
06/23/23Gene was removed from 'from '---' to ' VanC1''
06/06/23MLST was updated: from '---' to 'ST17(Pasteur)'
06/05/23Gene was added to 'eat(A)'
06/05/23Category was added: 'Efflux pumps/Other'
06/05/23Gene was added to 'sat4'
06/05/23Gene was added to 'dfrG'
06/05/23Gene was added to 'tet(L)'
06/05/23Gene was added to 'VanC1'
06/05/23Category was added: 'Streptothricin'
06/05/23Category was added: 'Trimethoprim'
06/05/23Category was added: 'Tetracyclines'
06/05/23Gene was removed from 'from '---' to ' VanB''
06/05/23Gene was added to 'aph(3')-IIIa'
06/05/23Gene was added to 'ant(6)-Ia'
06/05/23Gene was added to 'aacA-ENT1'
06/05/23Gene was added to 'aac(6')-Ie'
06/05/23Category was added: 'Aminoglycoside'
06/02/23Drug was removed: 'Quinupristin/dalfopristin'
11/20/18New Isolate added to panel
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