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Panel:   Tedizolid/Linezolid (AR-0781 - 0795) and VRE (AR-0474 - 0488) (Custom)
AR Bank # 0786 Enterococcus faecalis
Study ID: VRE-06

Biosample Accession #: SAMN15040085

   
MLST: ST6(Pasteur)


Panel:  Vancomycin-Resistant Enterococci (VRE)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Ampicillin 0.5S
Chloramphenicol 16I
Daptomycin 0.5S
Doxycycline <=1S
High-Level Gentamicin 1>500SYN-R
High-Level Streptomycin 2<=1000SYN-S
Levofloxacin 0.5S
Linezolid 1S
Penicillin 1S
Quinupristin/dalfopristin 38R
Rifampin 2I
Tedizolid 0.25S
Teicoplanin 0.25S
Vancomycin 32R
SDD (Susceptible Dose Dependent)
S – I –R Interpretation (INT) derived from CLSI 2025 M100 S35
1 SYN-R: is not synergistic with cell-wall active agent(e.g., ampicillin, penicillin and vancomycin)
2 SYN-S: is synergistic with cell-wall active agent (e.g., ampicillin, penicillin and vancomycin) if the cell-wall active agent is also susceptible
3 Reflects MIC of the combination
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aac(6')-Ie
Efflux pumps/Other qacZ
Glycopeptides VanB
Macrolide-Lincosamide-Streptogramin erm(B), Isa(A), mph(D)
 
Disclaimer:
No disclaimer.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

Isolate History
Date Action Performed
02/29/24MLST was updated: from '---' to 'ST6(Pasteur)'
02/29/24Gene was added to Efflux pumps/Other: 'qacZ'
02/29/24Gene was added to Macrolide-Lincosamide-Streptogramin: 'mph(D)'
02/29/24Gene was added to Macrolide-Lincosamide-Streptogramin: 'Isa(A)'
02/29/24Gene was added to Macrolide-Lincosamide-Streptogramin: 'erm(B)'
02/29/24Gene was added to Aminoglycoside: 'aac(6')-Ie'
02/29/24Category was added: Efflux pumps/Other
02/29/24Category was added: Macrolide-Lincosamide-Streptogramin
02/29/24Category was added: Aminoglycoside
11/20/23Sequence Accession # changed: from '---' to 'SAMN15040085'
02/14/23Isolate Status changed: from 'Pending' to 'Active'
02/14/23Drug was removed: 'Omadacycline'
02/14/23Isolate was removed from panel
02/14/23Isolate Status changed: from 'Active' to 'Pending'
02/14/23INT was updated with a new value for Tedizolid: from '---' to 'S'
02/14/23MIC was updated with a new value for Tedizolid: from '---' to '0.25'
02/14/23MIC was updated with a new value for Linezolid: from '2' to '1'
02/14/23Drug was added: Tedizolid
02/14/23MIC was updated with a new value for Tedizolid: from '---' to '0.25'
02/14/23MIC was updated with a new value for Linezolid: from '2' to '1'
02/14/23INT was updated with a new value for Omadacycline: from '---' to 'S'
02/14/23MIC was updated with a new value for Omadacycline: from '---' to '0.12'
02/14/23Drug was added: Omadacycline
11/20/18New Isolate added to panel
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