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Panel:   ARBank custom (n=29) (Custom)
AR Bank # 1048 Escherichia coli
Study ID: MEV-12

Biosample Accession #: SAMN16887362

   
MLST: 2(Pasteur), 617(Achtman)




Panel:  Meropenem/vaborbactam Verification (MEV)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Amikacin 2S
Ampicillin >32R
Ampicillin/sulbactam 1>32R
Aztreonam >64R
Cefepime >32R
Cefotaxime >64R
Cefotaxime/clavulanic acid 1>32---
Cefoxitin >16R
Ceftazidime 64R
Ceftazidime/avibactam 1<=0.5S
Ceftazidime/clavulanic acid 132---
Ceftolozane/tazobactam 1>16R
Ceftriaxone >32R
Ciprofloxacin >8R
Colistin 20.5I
Doripenem >8R
Ertapenem >8R
Gentamicin 0.5S
Imipenem >64R
Imipenem+chelators 4>32---
Levofloxacin 8R
Meropenem >8R
Meropenem-vaborbactam 1>16R
Minocycline <=4S
Piperacillin/tazobactam 1>128R
Tetracycline >32R
Tigecycline 3<=0.5S
Tobramycin 1S
Trimethoprim/sulfamethoxazole 1<=0.5S
S – I –R Interpretation (INT) derived from CLSI 2025 M100 S35
1 Reflects MIC of first component
2 Clinical and PK/PD data demonstrate colistin has limited clinical efficacy, even if an intermediate result is obtained. Alternative agents are strongly preferred. Colistin should be used in combination with one or more active antimicrobial agents. Consultation with an infectious disease specialist is recommended.
3 Based on FDA break points
4 Screen for metallo-beta-lactamase production [Rasheed et al. Emerging Infectious Diseases. 2013. 19(6):870-878]
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aph(3')-Ib, aph(6)-Id
Beta-lactam CTX-M-15, OXA-48
Sulfonamides sul2
Tetracyclines tet(B)
Truncated Porin OmpC, OmpC2, OmpK36
 
Disclaimer:
No disclaimer.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

Isolate History
Date Action Performed
12/12/22MLST was updated: from '---' to '617(Achtman)'
12/12/22MLST was updated: from '---' to '2(Pasteur)'
07/29/19New Isolate added to panel
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