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Panel:   ARBank custom (n=29) (Custom)
AR Bank # 1057 Escherichia coli
Study ID: MEV-21

Biosample Accession #: SAMN16887371

mCIM Result: Positive    
MLST: 866(Pasteur), 940(Achtman)




Panel:  Meropenem/vaborbactam Verification (MEV)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Amikacin >64R
Ampicillin >32R
Ampicillin/sulbactam 1>32R
Aztreonam 32R
Aztreonam/avibactam 1 34S
Cefepime >32R
Cefepime/zidebactam 50.5---
Cefotaxime >64R
Cefotaxime/clavulanic acid 1>32---
Cefoxitin >16R
Ceftazidime >128R
Ceftazidime/avibactam 1>16R
Ceftazidime/clavulanic acid 1>64---
Ceftolozane/tazobactam 1>16R
Ceftriaxone >32R
Ciprofloxacin >8R
Colistin 20.5I
Doripenem 8R
Ertapenem >8R
Gentamicin >16R
Imipenem 4R
Imipenem+chelators 40.5---
Levofloxacin >8R
Meropenem 8R
Meropenem-vaborbactam 18I
Minocycline >16R
Piperacillin/tazobactam 1>128R
Tetracycline >32R
Tigecycline 3<=0.5S
Tobramycin >16R
Trimethoprim/sulfamethoxazole 1>8R
S – I –R Interpretation (INT) derived from CLSI 2025 M100 S35
1 Reflects MIC of first component
2 Clinical and PK/PD data demonstrate colistin has limited clinical efficacy, even if an intermediate result is obtained. Alternative agents are strongly preferred. Colistin should be used in combination with one or more active antimicrobial agents. Consultation with an infectious disease specialist is recommended.
3 Based on FDA break points
4 Screen for metallo-beta-lactamase production [Rasheed et al. Emerging Infectious Diseases. 2013. 19(6):870-878]
5 Cefepime to zidebactam ratio (1:1)
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aac(3)-IId, aac(6')-IB3, aadA1, aph(3')-Ib, aph(3')-VI, aph(6)-Id, rmtC, sat-2A
Beta-lactam CMY-6, NDM-1, OXA-1, TEM-169
Macrolide-Lincosamide-Streptogramin erm(B)
Phenicols/Bicyclomycins catA1
Sulfonamides sul1, sul2
Tetracyclines tet(B)
Trimethoprim dfrA1
Truncated Porin OmpF
 
Disclaimer:
No disclaimer.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

Isolate History
Date Action Performed
03/20/25INT was updated with a new value for Aztreonam/avibactam: from '---' to 'S'
03/20/25MIC was updated with a new value for Aztreonam/avibactam: from '---' to '4'
03/20/25Drug was added: 'Aztreonam/avibactam'
12/12/22MLST was updated: from '---' to '940(Achtman)'
12/12/22MLST was updated: from '---' to '866(Pasteur)'
12/06/22MIC was updated with a new value for Cefepime/zidebactam: from '---' to '0.5'
12/06/22Drug was added: Cefepime/zidebactam
11/22/22mCIM changed: from '---' to 'Positive'
11/22/22INT was updated with a new value for Meropenem-vaborbactam: from 'S' to 'I'
11/22/22INT was updated with a new value for Imipenem: from 'I' to 'R'
11/22/22MIC was updated with a new value for Meropenem-vaborbactam: from '4' to '8'
11/22/22MIC was updated with a new value for Meropenem: from '4' to '8'
11/22/22MIC was updated with a new value for Imipenem: from '2' to '4'
07/29/19New Isolate added to panel
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